Metaproteome analysis reveals that syntrophy, competition, and phage-host interaction shape microbial communities in biogas plants
Metaproteome analysis reveals that syntrophy, competition, and phage-host interaction shape microbial communities in biogas plants
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DOI：
10.1186/s40168-019-0673-y
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发表时间：
2019-04-27
期刊：
影响因子：
15.5
通讯作者：
Reichl, U.
中科院分区：
文献类型：
--
作者：
Heyer, R.;Schallert, K.;Reichl, U.
背景在沼气厂中，复杂的微生物群落通过厌氧消化生物质来产生甲烷和二氧化碳。为了表征微生物的功能网络，使用高分辨率的元蛋白质组学流水线对11个反应器的样品进行了分析。结果受检的产甲烷古菌群落在与醋酸菌的共生过程中要么是混合营养的，要么是严格的水力遗传营养的。确定的代谢蛋白与厌氧消化模型1所描述的过程步骤的映射证实了其主要假设，并提出了一些扩展，如合养醋酸酯氧化或酒精发酵。结果表明，微生物群落是由共生、竞争和噬菌体-宿主相互作用形成的，导致细胞裂解。杆菌科、肠杆菌科和梭菌科的噬菌体数量超过宿主细胞的20倍。结论噬菌体诱导的细胞裂解可能会减缓底物向沼气的转化，但它可以通过营养物质的循环来支持营养缺陷型微生物的生长。
BackgroundIn biogas plants, complex microbial communities produce methane and carbon dioxide by anaerobic digestion of biomass. For the characterization of the microbial functional networks, samples of 11 reactors were analyzed using a high-resolution metaproteomics pipeline.ResultsExamined methanogenesis archaeal communities were either mixotrophic or strictly hydrogenotrophic in syntrophy with bacterial acetate oxidizers. Mapping of identified metaproteins with process steps described by the Anaerobic Digestion Model 1 confirmed its main assumptions and also proposed some extensions such as syntrophic acetate oxidation or fermentation of alcohols. Results indicate that the microbial communities were shaped by syntrophy as well as competition and phage-host interactions causing cell lysis. For the families Bacillaceae, Enterobacteriaceae, and Clostridiaceae, the number of phages exceeded up to 20-fold the number of host cells.ConclusionPhage-induced cell lysis might slow down the conversion of substrates to biogas, though, it could support the growth of auxotrophic microbes by cycling of nutrients.