The organisation of spinoparabrachial neurons in the mouse.
The organisation of spinoparabrachial neurons in the mouse.
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DOI：
10.1097/j.pain.0000000000000270
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发表时间：
2015-10
期刊：
影响因子：
7.4
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
这项研究表明，5%的板层I神经元的投射细胞，其中最表达的神经激肽1受体，这些通常可以区分基于其较大的尺寸从interneurons。前外侧束（ALT）起源于板层I和深背角中的神经元，代表主要的上行输出，伤害性信息通过该上行输出传递到涉及疼痛感知的脑区。虽然有详细的定量信息关于ALT在大鼠中，少得多的是知道这个系统在小鼠中，这是越来越多地被用于脊髓疼痛机制的研究，因为可用的转基因线。因此，本研究的目的是确定大鼠ALT相关信息可外推至小鼠的程度。我们的研究结果表明，在大鼠中，大多数椎板I ALT投射神经元在腰椎扩大可以逆行标记从外侧臂旁区，这些细胞（约90%）的大多数表达神经激肽1受体（NK1R），这些都大于其他NK1R表达神经元在这个椎板。这意味着，许多板I spinoparabrachial细胞可以确定在NK1r免疫染色切片从动物没有收到逆行示踪剂注射。然而，我们也观察到了某些物种的差异，特别是我们发现，在板层III和IV中的许多spinoparabrachial细胞缺乏NK1r，这意味着它们不能仅仅基于这种受体的表达来识别。我们还提供了证据表明，大多数spinoparabrachial细胞是apneumamatergic和一些表达P物质。这些研究结果将是重要的研究旨在解开复杂的神经元回路，脊髓疼痛处理的基础。
This study suggests that 5% of lamina I neurons are projection cells, which most express the neurokinin 1 receptor, and that these can generally be distinguished from interneurons based on their larger size. The anterolateral tract (ALT), which originates from neurons in lamina I and the deep dorsal horn, represents a major ascending output through which nociceptive information is transmitted to brain areas involved in pain perception. Although there is detailed quantitative information concerning the ALT in the rat, much less is known about this system in the mouse, which is increasingly being used for studies of spinal pain mechanisms because of the availability of genetically modified lines. The aim of this study was therefore to determine the extent to which information about the ALT in the rat can be extrapolated to the mouse. Our results suggest that as in the rat, most lamina I ALT projection neurons in the lumbar enlargement can be retrogradely labelled from the lateral parabrachial area, that the majority of these cells (∼90%) express the neurokinin 1 receptor (NK1r), and that these are larger than other NK1r-expressing neurons in this lamina. This means that many lamina I spinoparabrachial cells can be identified in NK1r-immunostained sections from animals that have not received retrograde tracer injections. However, we also observed certain species differences, in particular we found that many spinoparabrachial cells in laminae III and IV lack the NK1r, meaning that they cannot be identified based solely on the expression of this receptor. We also provide evidence that the majority of spinoparabrachial cells are glutamatergic and that some express substance P. These findings will be important for studies designed to unravel the complex neuronal circuitry that underlies spinal pain processing.