Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis
Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis
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DOI：
10.1093/jn/132.1.11
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发表时间：
2002-01-01
影响因子：
4.2
通讯作者：
Gil, A
中科院分区：
文献类型：
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作者：
Nieto, N;Torres, MI;Gil, A
本研究的目的是确定饮食中摄入（n-3）和（n-6）系列的单不饱和脂肪酸（MUFA）和/或多不饱和脂肪酸（PUFA）是否可以改善实验性溃疡性结肠炎（UC）的肠道损伤并减少炎症。大鼠接受 80 mg/kg 体重的 2,4,6-三硝基苯磺酸治疗，并喂食富含橄榄油 (00)、鱼油 (FO) 或纯化猪脑磷脂 (BPL) 的饮食 1 或 2 周，作为 (n-3) 和 (n-3) + (n-6) 系列单不饱和脂肪酸和 PUFA 的来源。对宏观和微观结肠损伤进行评估。分析了超微结构和组织学变化以及血浆和结肠粘膜脂肪酸谱以及损伤和炎症的一些生化标志物[碱性磷酸酶（AP）、米尔过氧化物酶（MPO）、前列腺素E-2（PGE（2））和白三烯B-4]。血浆和粘膜的脂肪酸谱主要反映了膳食脂肪酸组成。第 1 周和第 2 周，饲喂 00 饮食的 UC 动物的血浆 MUFA 比例高于 FO 或 BPL 组，并且 FO 中的 (n-3) 长链 PUFA (LC-PUFA) 高于 00 和 BPL 组。 1 周时，与 00 组和 BPL 组相比，FO 组的 UC 导致 MUFA 粘膜水平降低，并且 (n-3)LC-PUFA 更高。 1 周后喂食 FO 的 UC 大鼠表现出明显较少的宏观和微观结肠损伤。与 00 和 BPL 组相比，它们的 AP 和 MPO 活性以及 PGE(2) 水平也较低，并且组织学修复增强，粘膜内坏死区域较少，并且具有更多具有成熟粘蛋白颗粒的杯状细胞。这些结果表明，使用含有 (n-3) LC-PUFA 的均衡饮食可以改善 UC 的炎症和粘膜损伤。
The aim of the present study was to determine whether dietary intake of monounsaturated (MUFA) and/or polyunsaturated fatty acids (PUFA) of the (n-3) and (n-6) series could improve intestinal damage and reduce inflammation in experimental ulcerative colitis (UC). Rats were treated with 80 mg/kg body of 2,4,6-trinitrobenzenesulfonic acid and fed for 1 or 2 wk diets enriched in olive oil (00), fish oil (FO), or purified pig brain phospholipids (BPL), as sources of monounsaturated and PUFA of the (n-3) and (n-3) + (n-6) series. Evaluation of macroscopic and microscopic colonic damage was assessed. Ultrastructural and histologic changes were analyzed as well as plasma and colonic mucosa fatty acid profiles and some biochemical markers of injury and inflammation [alkaline phosphatase (AP), mieloperoxidase (MPO), prostaglandin E-2 (PGE(2)) and leukotriene B-4]. Fatty acid profiles of both plasma and mucosa mostly reflected the dietary fatty acid composition. Plasma MUFA proportions were higher in UC animals fed the 00 diet compared with FO or BPL groups 1 and 2 wk and (n-3) long chain PUFA (LC-PUFA) were higher in the FO than in the 00 and BPL groups. At 1 wk, UC led to lower MUFA mucosa levels and (n-3)LC-PUFA were higher in the FO group compared with the 00 and BPL groups. Rats with UC fed FO at 1 wk showed significantly less macroscopic and microscopic colonic damage. They also have lower AP and MPO activities and PGE(2) levels compared with the 00 and BPL groups and showed enhanced histological repair, less necrotic areas within the mucosa, and more goblet cells with mature mucin granules. These results suggest that the use of balanced diets containing (n-3) LC-PUFA could ameliorate the inflammation and mucosal damage in UC.