Cyclic hydrostatic pressure enhances the chondrogenic phenotype of human mesenchymal progenitor cells differentiated in vitro
Cyclic hydrostatic pressure enhances the chondrogenic phenotype of human mesenchymal progenitor cells differentiated in vitro
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DOI：
10.1016/s0736-0266(02)00230-9
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发表时间：
2003-05-01
影响因子：
2.8
通讯作者：
Johnstone, B
中科院分区：
文献类型：
--
作者：
Angele, P;Yoo, JU;Johnstone, B
生物活性因子对间充质祖细胞分化和增殖的影响已受到广泛关注，但很少有研究探讨机械因素对这些细胞的作用。本研究考察了循环静水压力对正在进行软骨分化的人骨髓来源间充质祖细胞的影响。骨髓来源间充质祖细胞聚集体在特定的软骨形成培养基中培养，并承受循环静水压力。聚集体在不同时间点加载：单次（第1天或第3天）或多次（第1 - 7天）。在第14天和第28天，收集聚集体进行组织学、免疫组织化学以及定量DNA和基质大分子分析。与未加载的对照组相比，加载一天的聚集体在蛋白聚糖和胶原蛋白含量上没有显示出显著变化。相反，对于多天加载的聚集体，在第14天和第28天均发现蛋白聚糖和胶原蛋白含量有统计学意义的增加。加载7天的聚集体更大，组织学染色显示基质/细胞比例更高。本研究表明静水压力可促进体外分化的间充质祖细胞的软骨基质形成，并提示机械力可能在体内软骨修复和再生中起重要作用。（C）2003骨科研究学会。由爱思唯尔科学有限公司出版。保留所有权利。
Much attention has been given to the influences of bioactive factors on mesenchymal progenitor cell differentiation and proliferation, but few studies have examined the effect of mechanical factors on these cells. This study examined the effects of cyclic hydrostatic pressure on human bone marrow-derived mesenchymal progenitor cells undergoing chondrogenic differentiation. Aggregates of bone marrow-derived mesenchymal progenitor cells were cultured in a defined chondrogenic medium and were subjected to cyclic hydrostatic pressure. Aggregates were loaded at various time points: single (day 1 or 3) or multiple (days 1-7). At 14 and 28 days, aggregates were harvested for histology, immunohistochemistry, and quantitative DNA and matrix macromolecule analysis. The aggregates loaded for a single day did not demonstrate significant changes in proteoglycan and collagen contents compared with the non-loaded controls. In contrast, for the multi-day loaded aggregates, statistically significant increases in proteoglycan and collagen contents were found on both day 14 and day 28. Aggregates loaded for seven days were larger and histological staining indicated a greater matrix/cell ratio. This study indicates that hydrostatic pressure enhances the cartilaginous matrix formation of mesenchymal progenitor cells differentiated in vitro, and suggests that mechanical forces may play an important role in cartilage repair and regeneration in vivo. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.