Development of Dynamic Liquid and Conjugated Electrospun Poly(L-lactide-co-caprolactone)/Collagen Nanoyarns for Regulating Vascular Smooth Muscle Cells Growth
Development of Dynamic Liquid and Conjugated Electrospun Poly(L-lactide-co-caprolactone)/Collagen Nanoyarns for Regulating Vascular Smooth Muscle Cells Growth
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用于调节血管平滑肌细胞生长的动态液体和共轭静电纺丝聚（L-丙交酯-共-己内酯）/胶原纳米纱的开发
DOI：
10.1166/jbn.2017.2352
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发表时间：
2017
影响因子：
2.9
通讯作者：
Mo Xiumei
中科院分区：
文献类型：
--
作者：
Wu Tong;Zhang Jialing;Wang Yuanfei;Sun Binbin;Guo Xuran;Morsi Yosry;El-Hamshary Hany;El-Newehy Mohamed;Mo Xiumei
模拟血管结构中的平滑肌层的建模对血管组织的再生有很大影响。功能性组织工程化血管基质将促进平滑肌细胞(SMC)向支架内的排列组织和三维穿透。为此，采用静电纺丝的方法，以无规纳米纤维和排列有序的纳米纤维为对照，制备了质量比为3：1的聚(L-丙交酯-己内酯)(P(LLA-CL))和胶原(COL)动态液体和共轭纳米纤维。傅里叶变换红外光谱和X射线衍射分析证实了P(LLA-CL)/COL的组成和结构保持不变。扫描电子显微镜结果表明，动态液体纤维的直径为19.27±6.16μm，共轭纤维的直径为10.24±3.09μm，两种纳米纤维的机械拉伸性能均优于无规纳米纤维。与随机排列的纳米纤维相比，纳米纤维具有更高的孔隙率和更大的孔径，导致水接触角减小，促进了SMCs的增殖和迁移。由于纱线微观结构的差异，共轭纳米纱具有较好的SMCs取向，而动态液态纳米纱具有较好的SMCs穿透性。因此，本研究证明了动态液电纺丝和共轭电纺法制备的定向多孔P(LLA-CL)/COL纳米丝有利于调节血管中膜细胞的生长，对血管中膜的功能重建具有重要意义。
Simulating the modeling of smooth muscle layer in the vascular structure makes a great difference for vascular tissue regeneration. A functional tissue engineered vascular media shall promote the aligned organization and three-dimensional penetration of smooth muscle cells (SMCs) into the scaffold. To this aim, dynamic liquid and conjugated nanoyarns based on poly(L-lactide-co-caprolactone) (P(LLA-CL)) and collagen (COL) with a weight ratio at 3:1 were fabricated by electrospinning methods, with random and aligned nanofibers as control groups. The Fourier transform infrared spectroscopy and X-ray diffraction analyses confirmed the preservation of P(LLA-CL)/COL components and structure. Scanning electron microscope (SEM) results indicated a significant increase of yarn diameters at 19.27 ± 6.16 μm (dynamic liquid) and 10.24 ± 3.09 μm (conjugated), and both of the nanoyarns had improved mechanical tensile properties than the random nanofibers. Compared with random and aligned nanofibers, the nanoyarns presented significant higher porosity and larger pore diameter, leading to a decrease of water contact angle and a promotion of SMCs proliferation and migration. Better SMCs orientation was observed on the conjugated nanoyarns, while superior SMCs penetration was achieved on the dynamic liquid nanoyarns, owing to the differences in yarns microstructure. Herein, this study demonstrated that the aligned and porous P(LLA-CL)/COL nanoyarns fabricated by dynamic liquid and conjugated electrospinning were beneficial to regulating vascular SMCs outgrowth, which had important implications for functional reconstruction of vascular media.