HLA-DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals.
HLA-DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals.
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HLA-DQA1 并不是各种自身免疫性疾病患者和健康个体中微嵌合现象的明显危险因素。
DOI：
10.1002/art.11235
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发表时间：
2003
影响因子：
--
通讯作者：
Rider,LisaG
中科院分区：
文献类型：
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作者：
Artlett,CarolM;O'Hanlon,TerrenceP;Lopez,AnaM;Song,YeongWook;Miller,FrederickW;Rider,LisaG
目的微嵌合细胞已在系统性硬化症（SSc）和特发性炎症性肌病（IIM）患者的病变和外周血中被发现，HLA-DQA 1 *0501是某些人群中这些疾病的危险因素。此外，DQA 1 *0501与SSc中的T淋巴细胞微嵌合体相关。为了更好地定义这种关联的强度，我们评估了DQA 1等位基因和microchimerization.MethodsDNA从整个外周血或磁分选T细胞microchimerization. DNA聚合酶链反应的Y染色体或HLA-Cw在87 SSc患者，28青少年IIM患者，和88名健康对照进行了测试。37对母子也进行了微嵌合体和DQA 1 * 0501的分析。结果我们无法证明DQA 1 *0501与SSc或青少年IIM患者或健康个体的T淋巴细胞或外周血DNA中的微嵌合体相关。在37对母子中，我们无法证明DQA 1 *0501与外周血DNA或T淋巴细胞中的微嵌合体相关，供受者HLA等位基因的相容性不影响受体的微嵌合体。DQA 1等位基因似乎没有发挥作用的持久性微嵌合体在外周血或T淋巴细胞的患者选定的自身免疫性疾病或健康个体。
ObjectiveMicrochimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymphocyte microchimerism in SSc. To better define the strength of this association, we assessed the relationship among DQA1 alleles and microchimerism.MethodsDNA from whole peripheral blood or magnetically sorted T cells was tested for microchimeric cells by polymerase chain reaction of the Y chromosome or of HLA–Cw in 87 SSc patients, 28 juvenile IIM patients, and 88 healthy controls. Thirty‐seven mother–son pairs were also analyzed for microchimerism and DQA1*0501.ResultsWe were unable to demonstrate that DQA1*0501 is associated with microchimerism in T lymphocytes or in whole peripheral blood DNA in patients with SSc or juvenile IIM or in healthy individuals. In the 37 mother–son pairs, we were unable to demonstrate an association of DQA1*0501 with microchimerism in peripheral blood DNA or T lymphocytes, and compatibility between the donor's and recipient's HLA alleles did not influence microchimerism in the recipient.ConclusionThese data suggest that HLA–DQA1 alleles do not appear to play a role in the persistence of microchimerism in the peripheral blood or T lymphocytes of patients with selected autoimmune diseases or in healthy individuals.