Identification of Suitable Reference Genes for Barley Gene Expression Under Abiotic Stresses and Hormonal Treatments
Identification of Suitable Reference Genes for Barley Gene Expression Under Abiotic Stresses and Hormonal Treatments
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鉴定非生物胁迫和激素处理下大麦基因表达的合适参考基因
DOI：
10.1007/s11105-014-0807-0
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发表时间：
2015-08-01
影响因子：
2.1
通讯作者：
Yang, Jianming
中科院分区：
文献类型：
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作者：
Hua, Wei;Zhu, Jinghuan;Yang, Jianming
大麦（Hordeum vulgare L.）是一种重要的谷类作物，也可以被认为是小麦族的模式植物。实时荧光定量PCR（qRT-PCR）是研究大麦基因表达的一种较为实用的技术，而与参考基因的数据归一化是获得准确可靠结果的关键。在这项研究中，17个候选参考基因进行了评估，从两个不同的植物组织（根和叶）在大麦苗期不同的非生物胁迫（渗透，盐，热）和激素处理（赤霉素A3（GA 3）和茉莉酸甲酯（JA））。我们的qRT-PCR数据用三种常用的软件包分析：geNorm、NormFider和BestKeeper。结合对这三个基因包的分析，在每个实验组中筛选出4 ~ 5个候选基因作为合适的参考基因。这些结果证实了参考基因的表达稳定性取决于实验条件。本研究对不同非生物和激素处理下大麦qRT-PCR上级参考基因的筛选进行了系统分析，为进一步研究大麦及小麦族其他物种的基因表达奠定了基础。
Barley (Hordeum vulgare L.) is an important cereal crop and also can be considered as a model plant for the Triticeae family. Quantitative real-time PCR (qRT-PCR) has emerged as a more practical technique to study barley gene expression, and the data normalization with reference genes is essential for accurate and reliable results. In this study, 17 candidate reference genes were evaluated from two distinct plant tissues (roots and leaves) at the barley seedling stage under different abiotic stresses (osmotic, salt, and heat) and hormonal treatments (gibberellin A3 (GA3) and methyl jasmonate (JA)). Our qRT-PCR data were analyzed with three commonly used software packages: geNorm, NormFider, and BestKeeper. In combination with the analysis of these three packages, four to five candidate genes were selected as suitable reference genes in each experimental set. These results confirmed that expression stability of reference genes depends on the experimental conditions. This study is the systematic analysis for the selection of superior reference genes for qRT-PCR in barley under different abiotic and hormonal treatments and will benefit further studies on gene expression in barley and other species of Triticeae family.