Dexamethasone suppresses mucus production and MUC-2 and MUC-5AC gene expression by NCI-H292 cells
Dexamethasone suppresses mucus production and MUC-2 and MUC-5AC gene expression by NCI-H292 cells
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DOI：
10.1152/ajplung.1996.271.3.l484
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发表时间：
1996-09-01
影响因子：
4.9
通讯作者：
Miyata, T
中科院分区：
文献类型：
--
作者：
Kai, H;Yoshitake, K;Miyata, T
气道粘液分泌过多是许多慢性炎症性肺病的特征。虽然目前针对粘液分泌过多的药理学方法有限，但糖皮质激素似乎是几种有用药物中最有效的。迄今为止，糖皮质激素对粘液产生的有效性的确切证据尚未得到充分阐明。本研究的目的是阐明地塞米松对人肺黏液表皮样癌细胞（NCI-H292）黏液产生和黏液蛋白基因表达的影响。NCI-H292细胞产生抗透明质酸酶的高分子量糖缀合物（HMWG），在Sepharose CL-4B柱层析中洗脱。地塞米松显著抑制NCI-H292细胞中[H-3]氨基葡萄糖或[H-3]丝氨酸标记的HMWG的基础生成。在Northern blot分析中，地塞米松降低了muc2和MUC-5AC粘蛋白基因的稳态mRNA水平。这些数据表明，地塞米松抑制气道黏液生成癌细胞中hmmwg的基础生成，并降低黏液蛋白基因的稳态mRNA水平。
Excessive production of airway mucus is a characteristic feature of many chronic inflammatory lung diseases. Although current pharmacological approaches to excessive mucus production are limited, glucocorticoids appear to be the most effective among a few useful drugs. The exact evidence for the effectiveness of glucocorticoids on mucus production has not been fully elucidated to date. The purpose of this study is to clarify the effect of dexamethasone on mucus production and mucin gene expression in a human pulmonary mucoepidermoid carcinoma cell line (NCI-H292). NCI-H292 cells produced hyaluronidase-resistant high-molecular-weight glycoconjugates (HMWG), which elute in the void volume on Sepharose CL-4B column chromatography. Dexamethasone significantly suppressed the basal production of [H-3]glucosamine- or [H-3]serine-labeled HMWG in NCI-H292 cells. In Northern blot analysis, dexamethasone attenuated steady-state mRNA levels of MUC-2 and MUC-5AC mucin genes. These data indicate that dexamethasone suppresses the basal production of HMWG and decreases steady-state mRNA levels of mucin genes in airway mucus-producing cancer cells.