Protective effect of ginsenosides Rg2 and Rh1 on oxidation-induced impairment of erythrocyte membrane properties
Protective effect of ginsenosides Rg2 and Rh1 on oxidation-induced impairment of erythrocyte membrane properties
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DOI：
10.3233/bir-2008-0516
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发表时间：
2008-01-01
期刊：
影响因子：
1.1
通讯作者：
Mitsuda, Noriaki
中科院分区：
文献类型：
--
作者：
Samukawa, Keiichi;Suzuki, Yoji;Mitsuda, Noriaki
据报道，人参提取物可改善各种器官的微循环。然而，这种现象背后的机制仍然知之甚少。本研究以红细胞流变学特性为指标，对人参提取物中的有效成分进行了筛选，确定了Rg（2）和Rh-1为人参提取物的有效成分。这两种皂甙可防止氧化应激引起的红细胞悬液粘度升高和红细胞对剪切应力的伸长反应。人参皂苷Rg 2和Rh 1在水相中不具有抗氧化活性，也不抑制膜脂过氧化。然而，他们抑制氧化诱导的减少SH-基团带3（阴离子交换器-1），红细胞膜的重要结构蛋白之一，但不是在其他结构蛋白：带1和2（血影蛋白），带4.2或带5（肌动蛋白）。这些结果表明，黄芪皂苷Rg 2和Rh 1通过防止带3蛋白中SH基团的氧化来保护红细胞的流变功能免受氧化应激。
The extract from Panax ginseng has been reported to improve the microcirculation in various organs. However, the mechanisms underlying this phenomenon are still poorly understood. In the present study, using the rheological properties of erythrocytes as an index, we have screened the components of Panax ginseng extract and identified Rg(2) and Rh-1 as the active ingredients. These two ginsenosides prevented the oxidative stress-induced elevation of erythrocyte suspension viscosity and the impairment of erythrocyte elongation in response to shear stress. Rg2 and Rh1 ginsenosides did not have antioxidant activity in an aqueous phase and did not inhibit the peroxidation of membrane lipids, either. However, they inhibited the oxidation-induced decrease of SH-groups in band 3 (anion exchanger-1), one of the important structural proteins of the erythrocyte membrane, but not in other structural proteins: bands 1 and 2 (spectrins), band 4.2 or band 5 (actin). These results suggest that ginsenosides Rg2 and Rh1 protect the rheological functions of erythrocytes against oxidative stress by preventing the oxidation of SH-groups in band 3 protein.