Reduced graphene oxide-coated electrospun fibre: effect of orientation, coverage and electrical stimulation on Schwann cells behavior
Reduced graphene oxide-coated electrospun fibre: effect of orientation, coverage and electrical stimulation on Schwann cells behavior
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还原氧化石墨烯涂层电纺纤维：定向、覆盖和电刺激对雪旺细胞行为的影响
DOI：
10.1039/d1tb00054c
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发表时间：
2021-03-21
影响因子：
7
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Huang, Zhiqiang;Sun, Manman;Li, Hong
电信号存在于神经细胞之间的细胞外间隙中。为了模拟周围神经再生的电生理环境，本研究旨在研究具有对齐拓扑结构的导电石墨烯基纤维支架如何通过电刺激（ES）调节体外许旺细胞行为。为此，随机和单轴排列的聚己内酯纤维支架通过静电纺丝，然后通过真空过滤用还原的氧化石墨烯（rGO）涂层制造。SEM显示rGO成功地涂覆在纤维上而不改变它们的取向，并且还带来了机械性能和亲水性的改善。rGO涂层纤维支架的电导率高达0.105 S m（-1）。当雪旺细胞接种在支架上并在体外受到10 mV的刺激时，发现纤维排列或ES导致雪旺细胞的增殖和神经生长因子（NGF）表达水平更高。此外，ES在对齐的纤维地形增强了神经生长因子的表达，雪旺细胞的增殖，并提高了细胞迁移率超过60%相比，无论是ES或单独的定向纤维。由模板生物材料介导的外源性电刺激的应用为神经再生提供了深刻的见解。
Electrical signals are present in the extracellular spaces between neural cells. To mimic the electrophysiological environment for peripheral nerve regeneration, this study was intended to investigate how conductive graphene-based fibrous scaffolds with aligned topography regulate Schwann cell behavior in vitro via electrical stimulation (ES). To this end, randomly- and uniaxially-aligned polycaprolactone fibrous scaffolds were fabricated by electrospinning, followed by coating with reduced graphene oxide (rGO) via vacuum filteration. SEM revealed that rGO was successfully coated on the fibers without changing their alignment, and also brought about an improvement in mechanical properties and hydrophilicity. The electrical conductivity of the rGO-coated fibrous scaffold was up to 0.105 S m(-1). When Schwann cells were seeded on the scaffolds and stimulated by 10 mV in vitro, it was found that either the alignment of the fibers or ES led to a higher level of proliferation and nerve growth factor (NGF) expression of Schwann cells. Further, ES at the aligned fibrous topography enhanced the expression of NGF, the proliferation of Schwann cells, and enhanced the cell migration rate by more than 60% compared to either ES or the oriented fibers alone. The application of exogenous electric cues mediated by templated biomaterials provides profound insights for nerve regeneration.