High abundance protein profiling of cystic fibrosis lung epithelial cells.
High abundance protein profiling of cystic fibrosis lung epithelial cells.
复制标题
囊性纤维化肺上皮细胞的高丰度蛋白质分析。
DOI：
10.1002/pmic.200401120
复制
发表时间：
2005
期刊：
影响因子：
3.4
通讯作者：
Jacobowitz,DavidM
中科院分区：
文献类型：
--
作者：
Pollard,HarveyB;Ji,Xiao-duo;Jozwik,Catherine;Jacobowitz,DavidM
Protein profiles of cultured cystic fibrosis (CF) lung epithelial cells were analyzed by two‐dimensional gel electrophoresis and mass spectrometry (MS). The analysis gave rise to a protein map over the pIrange of 4–7, and a molecular weight range ofca.100–10 kDa. The map contains 194 identified proteins, which were detectable by silver stain. All silver stained features were identified by matrix‐assisted laser desorption/ionization‐time of flight MS of tryptic peptides. Some proteins were found to be represented by multiple features on the 2‐D gel. Among the high abundance proteins identified were sets of proteins associated with inflammation, including the classical NFκB, p65 (RelA) and NFκB, p65 (RelB). We suggest that this composite atlas of the high abundance CF lung epithelial proteome will serve as a reference database for future studies of candidate CF drugs, validating different approaches to CFTR gene therapy, and analogous investigations of other types of human lung disorders.
