Nogo-A is a reliable oligodendroglial marker in adult human and mouse CNS and in demyelinated lesions
Nogo-A is a reliable oligodendroglial marker in adult human and mouse CNS and in demyelinated lesions
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DOI：
10.1097/01.jnen.0000248559.83573.71
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发表时间：
2007-03-01
影响因子：
3.2
通讯作者：
Brueck, Wolfgang
中科院分区：
文献类型：
--
作者：
Kuhlmann, Tanja;Remington, Leah;Brueck, Wolfgang
在组织切片中明确识别少突胶质细胞，特别是在有髓神经束中，往往是困难的。大多数用于识别少突胶质细胞的抗体也标记了髓鞘。Nogo-A最初被描述为轴突生长的抑制因子，已知在体内成熟的少突胶质细胞中强烈表达。在目前的研究中，我们分析了Nogo-A在成年小鼠和人中枢神经系统以及脱髓鞘动物模型和多发性硬化症皮损中的表达模式。将Nogo-A的表达与其他常用的少突胶质细胞标记物CC1、CNP和蛋白脂蛋白mRNA的原位杂交进行比较。Nogo-A在成人中枢神经系统和脱髓鞘病变中强而可靠地标记少突胶质细胞，因此是鉴定人和小鼠中枢神经组织中少突胶质细胞的有价值的工具。
The unambiguous identification of oligodendrocytes in tissue sections, especially in myelinated tracts, is often difficult. Most of the antibodies used to identify oligodendrocytes label the myelin sheath as well. Originally described as an inhibitor of axonal outgrowth, Nogo-A is known to be strongly expressed in mature oligodendrocytes in vivo. In the present investigation we analyzed the expression patterns of Nogo-A in adult mouse and human CNS as well as in demyelinating animal models and multiple sclerosis lesions. Nogo-A expression was compared with that of other frequently used oligodendroglial markers such as CC1, CNP, and in situ hybridization for proteolipid protein mRNA. Nogo-A strongly and reliably labeled oligodendrocytes in the adult CNS as well as in demyelinating lesions and thus represents a valuable tool for the identification of oligodendrocytes in human and mouse CNS tissue.