Selectivity of biopolymer membranes using HepG2 cells.
Selectivity of biopolymer membranes using HepG2 cells.
复制标题
使用 HepG2 细胞的生物聚合物膜的选择性
DOI：
10.1093/rb/rbu018
复制
发表时间：
2015-03
影响因子：
6.7
通讯作者：
Long M
中科院分区：
文献类型：
--
作者：
Lü D;Gao Y;Luo C;Lü S;Wang Q;Xu X;Sun S;Wang C;Long M
生物人工肝（BAL）系统已成为一种替代治疗的桥梁急性肝衰竭无论是肝移植或肝再生。目前的BAL系统在临床试验中效果不令人信服的主要原因之一是，作为BAL系统核心单元的肝生物反应器，其膜与肝细胞之间缺乏友好的界面。在这里，我们系统地比较了肝肉瘤HepG2细胞在8种市买生物相容性膜上的生物学反应，这些膜由乙酰纤维素-硝化纤维素混合纤维素（CA）、乙酰纤维素（CA）、尼龙（JN）、聚丙烯（PP）、硝化纤维素（NC）、聚氟乙烯（PVDF）、聚碳酸酯（PC）和聚四氟乙烯（PTFE）制成。物理化学分析和力学测试表明，CA、JN和PP膜具有较高的粘附性和合理的压缩和/或拉伸特性，具有适合细胞播种的表面形貌。细胞倾向于吸附在CA、JN、PP或PTFE膜上，呈球形，增殖率高。肌动蛋白（Actin）、白蛋白（albumin）和细胞角蛋白18 （cytokeratin 18）的表达有利于CA或PP膜上的细胞，而8种膜上的蛋白质过滤是一致的。这些结果进一步了解细胞的生长、形态和扩散，以及设计肝脏生物反应器时不同膜上的蛋白质过滤。
Bioartificial liver (BAL) system has emerged as an alternative treatment to bridge acute liver failure to either liver transplantation or liver regeneration. One of the main reasons that the efficacy of the current BAL systems was not convincing in clinical trials is attributed to the lack of friendly interface between the membrane and the hepatocytes in liver bioreactor, the core unit of BAL system. Here, we systematically compared the biological responses of hepatosarcoma HepG2 cells seeded on eight, commercially available biocompatible membranes made of acetyl cellulose–nitrocellulose mixed cellulose (CA–NC), acetyl cellulose (CA), nylon (JN), polypropylene (PP), nitrocellulose (NC), polyvinylidene fluoride (PVDF), polycarbonate (PC) and polytetrafluoroethylene (PTFE). Physicochemical analysis and mechanical tests indicated that CA, JN and PP membranes yield high adhesivity and reasonable compressive and/or tensile features with friendly surface topography for cell seeding. Cells prefer to adhere on CA, JN, PP or PTFE membranes with high proliferation rate in spheriod-like shape. Actin, albumin and cytokeratin 18 expressions are favorable for cells on CA or PP membrane, whereas protein filtration is consistent among all the eight membranes. These results further the understandings of cell growth, morphology and spreading, as well as protein filtration on distinct membranes in designing a liver bioreactor.