Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
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鹅、鸭、鸡分离的多种天布苏病毒株对雏鸭的实验感染效果
DOI：
10.24425/122613
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发表时间：
2018-01-01
影响因子：
0.8
通讯作者：
Li, Y.
中科院分区：
文献类型：
--
作者：
Xu, T.;Liu, X.;Li, Y.
为比较鹅、鸭、鸡坦布苏病毒（Tembusuvirus，TMUV）不同毒株的致病性，将56只5日龄樱桃谷雏鸭分为7组，分别以7 × 10 ~（5）PFU/ml的6种攻毒毒株肌肉注射感染。检测感染后1、3、5、7、11和14 dpi的临床体征、体重增加、死亡率、肉眼和显微镜病变、血清中的病毒载量和血清抗体滴度。结果表明，这些病毒均可引起雏鸭发病，但不同种源株的临床症状不同。与对照组相比，各试验组的增重均显著下降，但各组间增重及死鸭的肉眼和显微病变无明显差异。但水禽源毒株（鹅源和鸭源）的临床症状和血清中病毒载量的相对表达量均高于鸡源毒株。水禽组死亡率为37.5%，鸡组死亡率最高为12.5%。鹅群JS 804的血清抗体出现较早，效价较高。水禽源TMUV的致病性较鸡源TMUV强，JS 804可作为TMUV疫苗株。
In order to compare the pathogenicity of different Tembusu virus (TMUV) strains from geese, ducks and chickens, 56 5-day-old Cherry Valley ducklings which were divided into 7 groups and infected intramuscularly with 7 x 10(5) PFU/ml per duck of six challenge virus stocks. The clinical signs, weight gain, mortality, macroscopic and microscopic lesions, virus loads in sera of 1, 3, 5, 7, 11 and 14 dpi and serum antibody titers were examined. The results showed that these viruses could make the young ducks sick, but the clinical signs differed with the different species-original strains. All the experimental groups lose markedly in weight gain compared to the control, but there were no obvious distinctions in weight gains, as well as macroscopic and microscopic lesions of dead ducks between the infected groups. However, the groups of waterfowl-derived strains (from geese and ducks) showed more serious clinical signs and higher relative expressions of virus loads in sera than those from chicken-derived. The mortality of waterfowl groups was 37.5%, and the greatest mortality of chicken groups was 12.5%. The serum antibodies of the geese-species group JS804 appeared earlier and were higher in the titers than others. Taken toghter, the pathogenicity of waterfowl-derived TMUV was more serious than chicken-derived TMUV and JS804 could be chosen as one TMUV vaccine strain to protect from the infection.