Crude Fat, Diethyl Ether Extraction, in Feed, Cereal Grain, and Forage (Randall/Soxtec/Submersion Method): Interlaboratory Study, ISO/IUPAC/AOAC Harmonized Protocol
Crude Fat, Diethyl Ether Extraction, in Feed, Cereal Grain, and Forage (Randall/Soxtec/Submersion Method): Interlaboratory Study, ISO/IUPAC/AOAC Harmonized Protocol
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发表时间：
2003
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通讯作者：
N. Thiex;W. Adcock;J. Boedigheimer;E. Bogren;R. Coffin;K. Conway;A. DeBaker;E. Frankenius;B. Gildemeister;M. Gramse;P. Hogan;T. Knese;J. MacDonald;J. Moller;R. Royle;M. Russell;F. Shafiee;B. Schreve;J. Sieh;M. Spann;E. Topler;M. Watts
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作者：
N. Thiex;W. Adcock;J. Boedigheimer;E. Bogren;R. Coffin;K. Conway;A. DeBaker;E. Frankenius;B. Gildemeister;M. Gramse;P. Hogan;T. Knese;J. MacDonald;J. Moller;R. Royle;M. Russell;F. Shafiee;B. Schreve;J. Sieh;M. Spann;E. Topler;M. Watts
研究了动物饲料、谷物和草料（植物组织）中粗脂肪的测定方法。所提出的浸没法大大减少了完成一批样品所需的提取时间。吞吐量的增加提供了更快的周转时间，更高的劳动力使用效率和溶剂的回收。浸没法在肉类及肉制品油脂提取中已有研究，采用AOAC官方方法991.36。来自美国、瑞典、加拿大和德国的12位合作者对14份盲检样本进行了分析。粗脂肪的实验室内相对标准偏差（重复性）为1.09 ~ 9.26%。实验室间（包括实验室内部）相对标准偏差（再现性）范围为1.0 ~ 21.0%。该方法被推荐为AOAC国际第一行动官方方法。
A method for determining crude fat in animal feed, cereal grain, and forage (plant tissue) was collaboratively studied. The proposed submersion method considerably decreased the extraction time required to complete a batch of samples. The increase in throughput provided faster turnaround times, greater efficiency in the use of labor, and reclamation of the solvent. The submersion method for fat extraction was previously studied for meat and meat products and was accepted as AOAC Official Method 991.36. Fourteen blind samples were analyzed by 12 collaborators in the United States, Sweden, Canada, and Germany. The within-laboratory relative standard deviation (repeatability) ranged from 1.09 to 9.26% for crude fat. Among-laboratory relative (including within) relative standard deviation (reproducibility) ranged from 1.0 to 21.0%. The method is recommended as an AOAC INTERNATIONAL First Action Official Method.