A study on eggshell pigmentation: Biliverdin in blue-shelled chickens
A study on eggshell pigmentation: Biliverdin in blue-shelled chickens
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DOI：
10.1093/ps/85.3.546
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发表时间：
2006-03-01
期刊：
影响因子：
4.4
通讯作者：
Yang, N
中科院分区：
文献类型：
--
作者：
Zhao, R;Xu, GY;Yang, N
胆绿素是鸡和其他鸟类蛋壳中的重要色素。确定胆绿素的生物合成位点对于了解蛋壳色素沉着的生化过程和遗传基础至关重要。血液或蛋壳腺都可能是蛋壳胆绿素的生物合成位点。在中国本土鸡品种中构建了一个分离群体，其全同胞姐妹基因型为 Oo 和 oo，分别产蓝壳蛋和浅棕色蛋。采用紫外分光光度法和高效液相色谱法测定两组鸡蛋壳、血液、胆汁、排泄物、壳腺中胆绿素的浓度。蓝壳鸡和棕壳鸡蛋壳中胆绿素含量差异显着（P < 0.01）。产卵前3～4小时检测血液和胆汁，随机检测排泄物。结果显示，两组之间血液、胆汁和排泄物中胆绿素浓度无显着差异。蓝壳鸡和棕壳鸡壳腺中胆绿素含量分别为8.25±2.55和1.29±0.12 nmol/g，差异显着（P < 0.01）。我们的结果表明，血液不是壳胆绿素的生物合成位点。胆绿素很可能是在壳腺中合成的，然后沉积在鸡的蛋壳上。
Biliverdin is an important pigment in the eggshell of chickens and other avian species. Determination of the biosynthesis site for biliverdin is essential for understanding the biochemical process and genetic basis of eggshell pigmentation. Either blood or the shell gland could be the biosynthesis site of eggshell biliverdin. A segregation population with full-sib sisters genotyped Oo and oo, which laid blue-shelled eggs and light brown eggs, respectively, was constructed in a native Chinese chicken breed. Ultraviolet spectrophotometry and HPLC were used to determine the biliverdin concentration in eggshells, blood, bile, excreta, and shell gland of both groups of chickens. Biliverdin content was significantly different between egg shells of blue-shelled and brown-shelled chickens (P < 0.01). Blood and bile were tested 3 to 4 h before oviposition, and excreta was tested randomly. Results showed no significant difference in biliverdin concentration in blood, bile, and excreta between the 2 groups. In the shell gland, the biliverdin contents for the blue-shelled and brown-shelled chickens were 8.25 +/- 2.55 and 1.29 +/- 0.12 nmol/g, respectively, which showed a significant difference (P < 0.01). Our results demonstrated that blood is not the biosynthesis site of the shell biliverdin. Biliverdin is most likely synthesized in the shell gland and then deposited onto the eggshell of chickens.