Temporal transcriptomics suggest that twin-peaking genes reset the clock
Temporal transcriptomics suggest that twin-peaking genes reset the clock
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DOI：
10.7554/elife.10518
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发表时间：
2015-11-02
期刊：
影响因子：
7.7
通讯作者：
Oliver, Peter L.
中科院分区：
文献类型：
--
作者：
Pembroke, William G.;Babbs, Arran;Oliver, Peter L.
哺乳动物视交叉上核(SCN)驱动日常的节律性行为和生理，但对其协调转录程序缺乏详细的了解。为了揭示哺乳动物SCN转录组中昼夜节律变化的更详细的细节，我们结合了激光捕获显微切割(LCM)和RNA-SEQ在24小时的光/暗周期中。我们发现，与先前在SCN中观察到的基因相比，有7倍以上的基因表现出典型的正弦表达特征。另一组由766个基因组成的组出人意料地出现了两次峰值，在黑暗阶段的开始和结束附近；这组双峰组的突触传输基因显著丰富，而突触传输基因对于光诱导生物钟的相移至关重要。341个基因间非编码RNAs和包括Cry1在内的新的蛋白质编码基因外显子也显示出特定的昼夜节律表达变异。总体而言，我们的数据提供了一个重要的时间生物学资源(www.wgpembroke.com/shiny/SCNseq/)，并使我们能够提出SCN中的转录时序正在门控时钟重置机制。
The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light /dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN. Another group of 766 genes unexpectedly peaked twice, near both the start and end of the dark phase; this twin-peaking group is significantly enriched for synaptic transmission genes that are crucial for light-induced phase shifting of the circadian clock. 341 intergenic non-coding RNAs, together with novel exons of annotated protein-coding genes, including Cry1, also show specific circadian expression variation. Overall, our data provide an important chronobiological resource (www.wgpembroke.com/shiny/SCNseq/) and allow us to propose that transcriptional timing in the SCN is gating clock resetting mechanisms.