PHOSPHORYLATION OF VERY LYSINE-RICH HISTONE IN PHYSARUM-POLYCEPHALUM - CORRELATION WITH CHROMOSOME CONDENSATION
PHOSPHORYLATION OF VERY LYSINE-RICH HISTONE IN PHYSARUM-POLYCEPHALUM - CORRELATION WITH CHROMOSOME CONDENSATION
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DOI：
10.1111/j.1432-1033.1973.tb02664.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
SARNER, N
中科院分区：
文献类型：
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作者：
BRADBURY, EM;INGLIS, RJ;SARNER, N
已通过丙烯酰胺凝胶电泳分析了绒泡菌多头组蛋白。五个主要条带中的两个具有与来自小牛胸腺组蛋白的两个条带相同的电泳迁移率。 TheP。多头模式在同步有丝分裂周期的所有阶段都是相同的。据报道，富含赖氨酸的组蛋白的相对比例和相对迁移率发生了定量变化。特别是，该组蛋白的磷酸盐含量显着增加发生在 G2 期晚期，在相差显微镜下观察到出现染色体浓缩的峰值。 S期和RNA合成高峰期间磷酸盐含量较低。
Physarum polycephalumhistones have been analysed by acrylamide gel electrophoresis. Two of the five major bands had electrophoretic mobilities identical with the mobilities of two bands from calf thymus histone. TheP. polycephalumpattern is qualitatively the same at all stages of the synchronous mitotic cycle. Quantitative changes in the relative proportion and relative mobility of the very‐lysine‐rich histone are reported. In particular, a dramatic increase in phosphate content of this histone occurred in late G2 phase with a peak where chromosome condensation is seen to be occurring in the phase contrast microscope. Phosphate content is low during S phase and the peaks of RNA synthesis.