Within-subject test-retest reliability of the atlas-based cortical volume measurement in the rat brain: A voxel-based morphometry study
Within-subject test-retest reliability of the atlas-based cortical volume measurement in the rat brain: A voxel-based morphometry study
复制标题
大鼠大脑中基于图集的皮质体积测量的受试者内重测可靠性：基于体素的形态测量研究
DOI：
10.1016/j.jneumeth.2018.06.022
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发表时间：
2018-09-01
影响因子：
3
通讯作者：
Xu, Zhi-Qing David
中科院分区：
文献类型：
--
作者：
Jing, Bin;Liu, Bo;Xu, Zhi-Qing David
背景资料：各种神经和心理障碍与特定大脑区域的皮质体积变化有关，这可以使用结构磁共振成像（sMRI）在体内测量。使用大鼠模型进行MRI研究的兴趣越来越大，特别是在脑疾病和药物干预的纵向研究中。然而，在sMRI中观察到的形态学变化只有在测量结果可靠时才有意义。迄今为止，对大鼠脑组织形态计量学测量的重测信度的系统评价尚缺乏。新方法：我们严格评价了基于体素的形态计量学（VBM）分析的形态计量学测量的重测信度。37只SD大鼠每隔6小时扫描两次，并使用VBM-DARTEL方法估计灰质体积。结果：大部分脑区在6 h内具有良好的重测信度，少数脑区的重测信度较低，尤其是压后颗粒皮质。与现有方法的比较：据我们所知，这是第一个研究，以量化的重测信度的VBM测量的大鼠brain.Conclusions基于大脑皮层体积的VBM-DARTEL方法可以可靠地估计在大多数亚区。然而，必须谨慎解释可靠性较低的次区域的调查结果。
Background: Various neurological and psychological disorders are related to cortical volume changes in specific brain regions, which can be measured in vivo using structural magnetic resonance imaging (sMRI). There is an increasing interest in MRI studies using rat models, especially in longitudinal studies of brain disorders and pharmacologic interventions. However, morphometric changes observed in sMRI are only meaningful if the measurements are reliable. To date, a systematic evaluation of the test-retest reliability of the morphometric measures in the rat brain is still lacking.New method: We rigorously evaluated the test-retest reliability of morphometric measures derived from the voxel-based morphometry (VBM) analysis. 37 Sprague-Dawley rats were scanned twice at an interval of six hours and the gray matter volume was estimated using the VBM-DARTEL method. The intraclass coefficient, percent volume change and Pearson correlation coefficient were used to evaluate the reliability in 96 subregions of the rat brain.Results: Most subregions showed excellent test-retest reliabilities within an interval of 6 h while a few regions demonstrated lower reliability, especially in the retrosplenial granular cortex. The results were consistent between different methods of reliability assessment.Comparison with existing method: To the best of our knowledge, this is the first study to quantify the test-retest reliability of the VBM measurements of the rat brain.Conclusions Atlas-based cortical volume of the rat brain can be reliably estimated using the VBM-DARTEL method in most subregions. However, findings in subregions with lower reliability must be interpreted with caution.