Antioxidant enzyme activities and the production of mda and 8-oxo-dG in chronic lymphocytic leukemia
Antioxidant enzyme activities and the production of mda and 8-oxo-dG in chronic lymphocytic leukemia
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DOI：
10.1016/s0891-5849(01)00521-4
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发表时间：
2001-06-01
影响因子：
7.4
通讯作者：
Sáez, GT
中科院分区：
文献类型：
--
作者：
Oltra, AM;Carbonell, F;Sáez, GT
慢性淋巴细胞白血病（CLL）是一种易受抗氧化酶改变和氧化应激影响的肿瘤性疾病。本文测定了慢性淋巴细胞白血病（CLL）患者淋巴细胞超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活性、氧化型谷胱甘肽/还原型谷胱甘肽（GSSG/GSH）比值、丙二醛（MDA）和8-氧代脱氧鸟苷（8-oxo-dG）含量，并与同龄正常人进行了比较。CLL淋巴细胞SOD、CAT活性降低，GPx活性升高。CLL淋巴细胞GSH含量增加，GSSG浓度保持不变。因此，获得了降低的GSSG/GSH比率。氧化产物MDA和损伤的DNA碱基8-oxo-dG在CLL中也增加。酶活性，GSSG/GSH比值，和MDA的观察到的变化显着增强，随着病程的增加，在几年。结果支持CLL淋巴细胞中的主要氧化应激状态，并强调检查参数作为疾病演变标志物的作用。(C)2001 Elsevier Science Inc.
Chronic lymphocytic leukemia (CLL) is a neoplastic disease susceptible to antioxidant enzyme alterations and oxidative stress. We have examined the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and the oxidized/reduced glutathione (GSSG/GSH) ratio together with the levels of malondialdehyde (MDA) and 8-oxo-2'-deoxyguanosine (8-oxo-dG) in lymphocytes of CLL patients and compared them with those of normal subjects of the same age. SOD and CAT activity decreased in CLL lymphocytes while GPx activity increased. GSH content of CLL lymphocytes also increased, and GSSG concentration remained constant. Thus, a reduced GSSG/GSH ratio was obtained. The oxidation product MDA, and the damaged DNA base 8-oxo-dG were also increased in CLL. The observed changes in enzyme activities, GSSG/GSH ratio, and MDA were significantly enhanced as the duration of the disease increased in years. The results support a predominant oxidative stress status in CLL lymphocytes and emphasize the role of the examined parameters as markers of the disease evolution. (C) 2001 Elsevier Science Inc.