Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
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DOI：
10.1016/j.fsi.2009.12.024
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发表时间：
2010-04-01
影响因子：
4.7
通讯作者：
Chim, Liet
中科院分区：
文献类型：
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作者：
Castex, Mathieu;Lemaire, Pierrette;Chim, Liet
研究了黑弧菌侵染下stylirostris对虾（Litopenaeus） 72 h的抗氧化防御和诱导氧化应激组织损伤。为此，首先用致病性V. nigripulchritudo菌株SFn1浸泡感染stylirostris，然后在感染后0、12、24、48和72 h （h.p.i）测定其消化腺的抗氧化防御能力：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（Gpx）、总抗氧化状态（TAS）、谷胱甘肽和诱导组织损伤（MDA和carbonyl蛋白）。同时测定血TAS。在攻毒过程中，通过测定黑氏弧菌的流行率和在虾血淋巴中的负荷来跟踪虾的感染水平。所有这些参数的变化在72马力。在10(7)CFU g（-1）的饲料中饲喂益生菌酸碱Pediococcus acidiactii MA18/5M一个月的对照虾和对虾进行了为期一个月的试验。我们的研究结果表明，浸泡V nigripulchritudo导致血淋巴感染在24h.p.i.时达到最大水平，而死亡高峰记录在48h.p.i.。从24h.p.i.开始检测到抗氧化防御的显著下降，超过这个时间感染导致氧化应激水平和组织损伤的增加。与对照组相比，食用益生菌饲料的对虾感染水平较低（24 hp时，对照组为20%，而不是45%），死亡率较低（25%，而不是41.7%）。此外，与未感染的对照虾相比，饲喂益生菌的感染虾表现出非常相似的抗氧化状态和氧化应激水平。与感染的对照组相比，喂食益生菌的对虾具有更高的抗氧化防御能力和更低的氧化应激水平。本研究表明，细菌感染导致柱头对虾氧化应激，并强调了P. acidilactici的有益作用，表明竞争排斥效应导致感染水平降低和/或增强对虾的抗氧化状态。(C) 2010 Elsevier Ltd.版权所有。
Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence and load in the haemolymph of the shrimps. Changes in all these parameters during the 72-h.p.i. period were recorded for control shrimps and shrimps previously fed for one month with probiotic Pediococcus aciditactici MA18/5M at 10(7) CFU g(-1) of feed.Our results showed that immersion with V nigripulchritudo led to maximal infection level in the haemolymph at 24 h.p.i. preceding the mortality peak recorded at 48 h.p.i. Significant decreases in the antioxidant defences were detected from 24 h.p.i. and beyond that time infection leaded to increases in oxidative stress level and tissue damage.. Compared to control group, shrimps fed the probiotic diet showed lower infection (20% instead of 45% at 24 h.p.i. in the control group) and mortality (25% instead of 41.7% in the control group) levels. Moreover, infected shrimp fed the probiotic compared to uninfected control shrimps exhibited very similar antioxidant status and oxidative stress level. Compared to the infected control group, shrimps fed the probiotic sustained higher antioxidant defences and lower oxidative stress level.This study shows that bacterial infection leads to oxidative stress in L. stylirostris and highlighted a beneficial effect of P. acidilactici, suggesting both a competitive exclusion effect leading to a reduction of the infection level and/or an enhancement of the antioxidant status of the shrimps. (C) 2010 Elsevier Ltd. All rights reserved.