Organization and expression of the COX6 genetic locus in Saccharomyces cerevisiae: multiple mRNAs with different 3' termini are transcribed from COX6 and regulated differentially.
Organization and expression of the COX6 genetic locus in Saccharomyces cerevisiae: multiple mRNAs with different 3' termini are transcribed from COX6 and regulated differentially.
复制标题
酿酒酵母中COX6基因座的组织和表达：具有不同3末端的多个mRNA从COX6转录并进行差异调节。
DOI：
10.1093/nar/17.3.1103
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发表时间：
1989
影响因子：
14.9
通讯作者：
Poyton,RO
中科院分区：
文献类型：
--
作者：
Wright,RM;Rosenzweig,B;Poyton,RO
酿酒酵母的COX6及其周围的遗传位点已被鉴定。在COX6的上游和下游发现了紧密分布的侧翼基因。上游基因和COX6从相反的链转录而来，相隔不超过300个碱基对。COX6在其3‘非翻译区转录成三种不同大小的信使核糖核酸(1000b、830b和700b)，长度不同。所有三类mRNAs都在多聚体上发现，因此很可能是翻译的。不同的COX6 mRNAs在富含糖的培养基中生长过程中丰度不同，随着细胞被转移到高或低葡萄糖浓度的培养基中，不同的COX6 mRNA受到不同的影响。最大的信使核糖核酸比两个较小的信使核糖核酸更容易受到葡萄糖抑制/去抑制的影响，而最小的信使核糖核酸优先在富营养液中积累。这些发现表明，具有不同3‘末端的COX6mRNAs要么是差异合成的，要么是稳定性不同的，提示存在一个复杂的系统来调节COX6的表达。
COX6 and its surrounding genetic locus have been characterized for the yeastSaccharomyces cerevisiae. Flanking genes are found closely spaced upstream and downstream of COX6. The upstream gene and COX6 are transcribed from opposite strands and are separated by no more than 300 bp. COX6 is transcribed into three different size classes of mRNA (1000b, 830b, and 700b) differing in length in their 3' untranslated regions. All three classes of mRNAs are found on polysomes and, hence, are most likely translated. The different COX6 mRNAs vary in abundance during growth in rich media and are affected differentially as cells are shifted into media containing high or low glucose concentrations. The largest mRNA is much more susceptible to glucose repression/derepression than are the two smaller mRNAs, whereas the smallest RNA is preferentially accumulated during growth in rich media. These findings demonstrate that COX6 mRNAs with different 3'-termini are either synthesized differentially or differ in stability and suggest the existence of a complex system regulating COX6 expression.