The nodal pathway acts upstream of hedgehog signaling to specify ventral telencephalic identity
The nodal pathway acts upstream of hedgehog signaling to specify ventral telencephalic identity
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DOI：
10.1016/s0896-6273(01)00210-0
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发表时间：
2001-02-01
期刊：
影响因子：
16.2
通讯作者：
Wilson, SW
中科院分区：
文献类型：
--
作者：
Rohr, KB;Barth, KA;Wilson, SW
Nodal和Hedgehog信号通路影响CNS所有轴向水平的背腹模式，但这些信号通路如何相互作用来调节模式在很大程度上仍不清楚。在这里，我们表明，在斑马鱼中，节点信号是诱导同源框基因nk2所必需的。1a在腹侧间脑，nk2.1b在腹侧端脑。端脑nk2也需要Hedgehog信号。1b的表达，但可能不是建立间脑nk2所必需的。LA表情。此外，Shh不能恢复缺乏结节活动的胚胎的腹侧间脑发育。相反，Shh确实在没有Nodal活动的情况下恢复了端脑nk2.1b的表达，这表明Hedgehog信号作用于Nodal活动的下游，形成腹侧端脑的模式。因此，Nodal通路通过Hedgehog信号依赖和非依赖机制来调节腹侧前脑模式。
The Nodal and Hedgehog signaling pathways influence dorsoventral patterning at all axial levels of the CNS, but it remains largely unclear how these pathways interact to mediate patterning. Here we show that, in zebrafish, Nodal signaling is required for induction of the homeobox genes nk2. 1a in the ventral diencephalon and nk2.1b in the ventral telencephalon. Hedgehog signaling is also required for telencephalic nk2. 1b expression but may not be essential to establish diencephalic nk2. la expression. Furthermore, Shh does not restore ventral diencephalic development in embryos lacking Nodal activity. In contrast, Shh does restore telencephalic nk2.1b expression in the absence of Nodal activity, suggesting that Hedgehog signaling acts downstream of Nodal activity to pattern the ventral telencephalon. Thus, the Nodal pathway regulates ventral forebrain patterning through both Hedgehog signaling-dependent and -independent mechanisms.