MITOCHONDRIAL-DNA SEQUENCES IN THE NUCLEAR GENOME OF A LOCUST
MITOCHONDRIAL-DNA SEQUENCES IN THE NUCLEAR GENOME OF A LOCUST
复制标题
DOI：
10.1038/301631a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
WYATT, GR
中科院分区：
文献类型：
--
作者：
GELLISSEN, G;BRADFIELD, JY;WYATT, GR
线粒体起源的内共生理论1被广泛接受，并意味着在进化过程中，真核细胞的基因从线粒体到细胞核的丢失已经发生2，3。然而，这些细胞器之间的基因转移在DNA序列水平上的证据迄今为止仅限于一个例子，即粗糙脉孢菌的线粒体ATP酶亚基基因在核基因组中有一个同源伴侣4。从昆虫的基因文库中，飞蝗，我们现在已经分离出两个克隆，代表不同的片段的核DNA，其中包含的序列同源的线粒体基因的核糖体RNA，以及高度重复的核序列的同源性区域。结果表明，线粒体和核基因组之间的序列转移，其次是进化分歧。
The endosymbiotic theory1of the origin of mitochondria is widely accepted, and implies that loss of genes from the mitochondria to the nucleus of eukaryotic cells has occurred over evolutionary time2,3. However, evidence at the DNA sequence level for gene transfer between these organelles has so far been limited to a single example, the demonstration that a mitochondrial ATPase subunit gene ofNeurospora crassahas an homologous partner in the nuclear genome4. From a gene library of the insect,Locusta migratoria, we have now isolated two clones, representing separate fragments of nuclear DNA, which contain sequences homologous to the mitochondrial genes for ribosomal RNA, as well as regions of homology with highly repeated nuclear sequences. The results suggest the transfer of sequences between mitochondrial and nuclear genomes, followed by evolutionary divergence.