Imaging of macrophage dynamics with optical coherence tomography in anterior ischemic optic neuropathy
Imaging of macrophage dynamics with optical coherence tomography in anterior ischemic optic neuropathy
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DOI：
10.1016/j.exer.2016.11.020
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发表时间：
2017-01-01
影响因子：
3.4
通讯作者：
Zinkernagel, Martin S.
中科院分区：
文献类型：
--
作者：
Kokona, Despina;Haner, Nathanael U.;Zinkernagel, Martin S.
前部缺血性视神经病变(AION)是一种相对常见的视力丧失的原因，是由于视神经前部的小动脉灌流不足所致。AION是突发性视神经相关性视力丧失的主要原因，在50岁以上的人群中，每10万人中约有10例。到目前为止，AION还没有确定的治疗方法，因此更好地了解发生在视神经头(ONH)水平上的事件对于设计未来的治疗策略将是重要的。眼睛的光学特性允许在缺血期间对视神经进行体内成像，视神经是中枢神经系统的一部分。实验研究表明，激光诱导的视神经病变与人类前部缺血性视神经病变具有相似的解剖学特征。在激光诱导的视神经病变后，我们发现光学相干断层扫描中的高反射点与组织学上的单核细胞相对应。利用荧光激活的流式细胞术(FACS)，我们发现这些细胞在Elion后一周达到高峰。这些观察结果被转化为AION患者的OCT表现，在ONH发现了类似的高反射点动态。我们的数据表明，激活的巨噬细胞在OCT上可以被识别为高反射点。(C)2016年提交人。爱思唯尔有限公司出版。
Anterior ischemic optic neuropathy (AION) is a relatively common cause of visual loss and results from hypoperfusion of the small arteries of the anterior portion of the optic nerve. AION is the leading cause of sudden optic nerve related vision loss with approximately 10 cases per 100'000 in the population over 50 years. To date there is no established treatment for AION and therefore a better understanding of the events occurring at the level of the optic nerve head (ONH) would be important to design future therapeutic strategies. The optical properties of the eye allow imaging of the optic nerve in vivo, which is a part of the CNS, during ischemia. Experimentally laser induced optic neuropathy (eLiON) displays similar anatomical features as anterior ischemic optic neuropathy in humans. After laser induced optic neuropathy we show that hyperreflective dots in optical coherence tomography correspond to mononuclear cells in histology. Using fluorescence-activated flow cytometry (FACS) we found these cells to peak one week after eLiON. These observations were translated to OCT findings in patients with AION, where similar dynamics of hyperreflective dots at the ONH were identified. Our data suggests that activated macrophages can be identified as hyperreflective dots in OCT. (C) 2016 The Authors. Published by Elsevier Ltd.