RECEPTOR FOR MOUSE HEPATITIS-VIRUS IS A MEMBER OF THE CARCINOEMBRYONIC ANTIGEN FAMILY OF GLYCOPROTEINS
RECEPTOR FOR MOUSE HEPATITIS-VIRUS IS A MEMBER OF THE CARCINOEMBRYONIC ANTIGEN FAMILY OF GLYCOPROTEINS
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DOI：
10.1073/pnas.88.13.5533
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发表时间：
1991-07-01
影响因子：
11.1
通讯作者：
HOLMES, KV
中科院分区：
文献类型：
--
作者：
WILLIAMS, RK;JIANG, GS;HOLMES, KV
小鼠肝炎病毒受体(MHV)是一种小鼠冠状病毒，是一种位于肠道刷状缘膜和肝细胞膜上的110-120 kDa的糖蛋白。免疫亲和纯化的MHV受体的N端25个氨基酸序列与两个与人癌胚抗原(CEA)相关的小鼠基因的成熟N端完全相同，并与人和大鼠CEA家族成员的N端高度同源。抗人CEA的多克隆抗体识别免疫亲和纯化的MHV受体和来自MHV敏感小鼠品系的肝膜和肠刷缘膜上的MHV受体。在耐MHV的SJL/J小鼠的膜上，抗CEA抗体识别一种未能结合MHV的同源糖蛋白。在BALB/c结肠、小肠和肝脏的膜上检测到MHV受体糖蛋白，这些膜是MHV在体内复制的主要靶点。MHV受体糖蛋白在分子质量、酸性等电点、广泛的糖基化、在高氯酸中的溶解性和组织分布方面与人类CEA家族的成员相似。因此，据我们所知，MHV受体是CEA糖蛋白家族中第一个被确定为病毒受体的成员。
The receptor for mouse hepatitis virus (MHV), a murine coronavirus, is a 110- to 120-kDa glycoprotein on intestinal brush border membranes and hepatocyte membranes. The N-terminal 25-amino acid sequence of immunoaffinity-purified MHV receptor was identical to the predicted mature N termini of two mouse genes related to human carcinoembryonic antigen (CEA) and was strongly homologous to the N termini of members of the CEA family in humans and rats. Polyclonal antibodies to human CEA recognized the immunoaffinity-purified MHV receptor and the MHV receptor in liver membranes and intestinal brush border membranes from MHV-susceptible mouse strains. In membranes from MHV-resistant SJL/J mice, the anti-CEA antibodies recognized a homologous glycoprotein that failed to bind MHV. The MHV receptor glycoprotein was detected in membranes of BALB/c colon, small intestine, and liver, which are the principal targets for MHV replication in vivo. The MHV receptor glycoprotein resembled members of the human CEA family in molecular weight, acidic pI, extensive glycosylation, solubility in perchloric acid, and tissue distribution. Thus, the MHV receptor is, to our knowledge, the first member of the CEA family of glycoproteins to be identified as a virus receptor.