Induction of the heat shock regulon does not produce thermotolerance in Escherichia coli.
Induction of the heat shock regulon does not produce thermotolerance in Escherichia coli.
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DOI：
10.1101/gad.1.6.525
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发表时间：
1987-08
影响因子：
10.5
通讯作者：
R. Vanbogelen;M. Acton;F. Neidhardt
中科院分区：
文献类型：
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作者：
R. Vanbogelen;M. Acton;F. Neidhardt
添加异丙基硫代-β-D-半乳糖苷（IPTG）的大肠杆菌细胞含有多个拷贝的热休克调节基因htpR（rpoH）的IPTG诱导型启动子（P-tac）的控制下，诱导15的17个多肽的热休克（HTP）调节子。感应的时间过程和幅度与42摄氏度的变化非常相似。然而，这两种诱导热休克调节子的方法在结果上是不同的。在28 ℃下生长并通过在42 ℃下孵育15分钟诱导的培养物对50 ℃的攻击温度提供了显著的保护，但在28 ℃下15分钟的IPTG处理没有提供保护。结果表明，IPTG对42 ℃耐热性的形成没有干扰作用。此外，还对E.大肠杆菌与各种有毒物质的研究表明，热耐受性的发展和热休克蛋白的任何子集的诱导之间没有相关性。热耐受性的发展过程以外的htpR依赖性诱导的热休克蛋白。
The addition of isopropyl thio-beta-D-galactoside (IPTG) to Escherichia coli cells containing multiple copies of the heat shock regulatory gene htpR (rpoH) under the control of an IPTG-inducible promoter (P-tac) induced 15 of the 17 polypeptides of the heat shock (HTP) regulon. The time course and magnitude of the induction closely resembled that caused by a shift to 42 degrees C. Nevertheless the two means of inducing the heat shock regulon differed in outcome. Cultures grown at 28 degrees C and induced by incubation at 42 degrees C for 15 min gave significant protection against a challenge temperature of 50 degrees C, but no protection was afforded by a 15-min IPTG treatment at 28 degrees C. It could be shown that there was no interference by IPTG with the development of thermotolerance at 42 degrees C. Also, treatment of a wild strain of E. coli with various toxic agents revealed no correlation between the development of thermotolerance and the induction of any subset of the heat shock proteins. Thermotolerance appears to develop by processes other than the htpR-dependent induction of heat shock proteins.