Role of macrophage migration inhibitory factor in ovalbumin-induced airway inflammation in rats
Role of macrophage migration inhibitory factor in ovalbumin-induced airway inflammation in rats
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DOI：
10.1183/09031936.06.00107004
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发表时间：
2006-04-01
影响因子：
24.3
通讯作者：
Nishimura, M
中科院分区：
文献类型：
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作者：
Kobayashi, M;Nasuhara, Y;Nishimura, M
巨噬细胞移动抑制因子（MIF）是一种促炎细胞因子，据报道可抵消内源性糖皮质激素的抗炎作用。只有少数报告表明MIF和支气管哮喘之间存在潜在联系。为了进一步阐明MIF在哮喘中的确切作用，本文作者在卵白蛋白免疫的大鼠哮喘模型中研究了抗MIF抗体（Ab）对气道炎症和气道高反应性的影响。支气管肺泡灌洗液中的MIF水平显着升高后，卵白蛋白challenge.免疫组化研究显示，即使在非致敏大鼠支气管上皮中的MIF免疫染色阳性，和支气管上皮中的MIF染色增强后，卵白蛋白challenge.抗MIF抗体能显著降低卵白蛋白诱导的大鼠支气管肺泡灌洗液中细胞总数、中性粒细胞和嗜酸性粒细胞的数量，并能减弱卵白蛋白诱导的气道高反应性。然而，抗-MIF抗体并不影响血清卵清蛋白特异性IgE的水平，这表明抗-MIF抗体并不抑制免疫本身。结果表明，巨噬细胞移动抑制因子在哮喘气道炎症和气道高反应性中起着至关重要的作用。
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that reportedly counteracts the anti-inflammatory effect of endogenous glucocorticoids. There have only been a few reports that demonstrate a potential link between MIF and bronchial asthma. In an attempt to further clarify the precise role of MIF in asthma, the present authors examined the effect of anti-MIF antibody (Ab) on airway inflammation and airway hyperresponsiveness in an ovalbumin-immunised rat asthma model.Actively immunised Brown Norway rats received ovalbumin inhalation with or without treatment of anti-MIF Ab. The levels of MIF in bronchoalveolar lavage fluid were significantly elevated after the ovalbumin challenge.An immunohistochemical study revealed positive immunostaining for MIF in bronchial epithelium, even in nonsensitised rats, and the MIF staining in bronchial epithelium was enhanced after the ovalbumin challenge. Anti-MIF Ab significantly decreased the number of total cells, neutrophils and eosinophils in the bronchoalveolar lavage fluid of the ovalbumin-challenged rats, and also attenuated the ovalbumin-induced airway hyperresponsiveness to ovalbumin and methacholine. However, anti-MIF Ab did not affect the level of serum ovalbumin-specific IgE, suggesting that anti-MIF Ab did not suppress immunisation itself.The results indicate that macrophage migration inhibitory factor plays a crucial role in airway inflammation and airway hyperresponsiveness in asthma.