A novel subgenotype I3 of hepatitis B virus in Guangxi, China: a 15-year follow-up study
A novel subgenotype I3 of hepatitis B virus in Guangxi, China: a 15-year follow-up study
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DOI：
10.1007/s11262-023-01980-6
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发表时间：
2023-02-25
期刊：
影响因子：
1.6
通讯作者：
Fang, Zhong-Liao
中科院分区：
文献类型：
--
作者：
Jia, Hui-Hua;Chen, Qin-Yan;Fang, Zhong-Liao
B型肝炎病毒（HBV）基因型I最近被提出后，从越南和老挝的HBV全基因组测序。然而，它的长期分子进化是未知的。本研究的目的是研究该基因型的分子进化，从一个无症状的HBsAg携带者从龙安队列超过15年的时间进行了研究，使用NGS和基于克隆的测序。2004年、2007年、2013年和2019年获得的全基因组序列数量分别为17、20、19和10。所有菌株都属于亚基因型I1，除了6株（5株来自2007年，1株来自2019年）和8株来自2007年的其他菌株，它们形成了从其他亚基因型I序列分支出来的聚类，得到了100%自助值的支持。基于全基因组序列，这些毒株与HBV亚型I1-I2之间的组内核苷酸差异值均超过4%。这些菌株是基因型I1和亚型C之间的重组体，但断点不同。该携带者中的宿主内病毒进化率中位数为每年每个位点3.88E-4替换。香农熵（Sn）的范围为0.55至0.88和遗传多样性，D，范围为0.0022至0.0041。总之，我们的数据提供了新的亚基因型的证据。考虑到这8株病毒在2007年后消失，而其中1株病毒在2019年再次出现，我们建议这6株病毒为一个新的亚型，暂命名为HBV亚型I3，8株病毒为异常基因型。
Genotype I of hepatitis B virus (HBV) was proposed recently following sequencing of complete HBV genomes from Vietnam and Laos. However, its long-term molecular evolution is unknown. The objectives of this study were to study the molecular evolution of this genotype from an asymptomatic HBsAg carrier from the Long An cohort over a 15-year period was studied using both NGS and clone-based sequencing. The number of complete genome sequences obtained in 2004, 2007, 2013, and 2019 are 17, 20, 19, and 10, respectively. All strains belong to subgenotype I1, except for six (five from 2007 and one from 2019) and 8 further strains from 2007 which form a cluster branching out from other subgenotype I sequences, supported by a 100% bootstrap value. Based on complete genome sequences, all of the estimated intragroup nucleotide divergence values between these strains and HBV subgenotypes I1-I2 exceed 4%. These strains are recombinants between genotype I1 and subgenotype C but the breakpoints vary. The median intrahost viral evolutionary rate in this carrier was 3.88E-4 substitutions per site per year. The Shannon entropy (Sn) ranged from 0.55 to 0.88 and the genetic diversity, D, ranged from 0.0022 to 0.0041. In conclusion, our data provide evidence of novel subgenotypes. Considering that the 8 strains disappeared after 2007, while one of the 6 strains appears again in 2019, we propose these 6 strains as a new subgenotype, provisionally designated HBV subgenotype I3 and the 8 strains as aberrant genotype.