Distribution and variable expression of secretory pathway protein reticulocalbin in normal human organs and non-neoplastic pathological conditions
Distribution and variable expression of secretory pathway protein reticulocalbin in normal human organs and non-neoplastic pathological conditions
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DOI：
10.1369/jhc.6a6943.2006
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发表时间：
2007-04-01
影响因子：
3.2
通讯作者：
Suzuki, Toshimitsu
中科院分区：
文献类型：
--
作者：
Fukuda, Takeaki;Oyamada, Hiroshi;Suzuki, Toshimitsu
网织红蛋白（Reticulocalbin，RCN）是分泌途径中的钙结合蛋白，定位于内质网。RCN可能在细胞的正常行为和生命中发挥作用，尽管其详细作用仍不清楚。RCN的过表达也可能在肿瘤发生、肿瘤侵袭和耐药性中起作用。人RCN的新抗体用于RCN在有或无炎症的胎儿和成人的正常人体器官中的分布。免疫组化结果显示，RCN广泛分布于胎儿和成人的各种器官，主要分布于内分泌和外分泌器官。然而，RCN的表达是异质性的，在每个组成细胞的一些器官。在非上皮器官中，血管内皮细胞、睾丸生殖细胞、神经元和滤泡树突状细胞显示强染色。浆细胞是造血和淋巴细胞中唯一的RCN阳性细胞。在炎症条件下，RCN表达增强上皮细胞和非上皮细胞。RCN的异质性表达表明，细胞行为和生命所需的RCN的量可能是可变的，这取决于每种细胞类型，因此，RCN可能有助于确定某些器官中肿瘤的细胞起源。然而，RCN在肿瘤发生中的意义和肿瘤的一些特殊特征还需要进一步的研究。
Reticulocalbin (RCN) is one member of the Ca2+-binding proteins in the secretory pathway and is localized in the endoplasmic reticulum. RCN may play a role in the normal behavior and life of cells, although its detailed role remains unknown. Overexpression of RCN may also play a role in tumorigenesis, tumor invasion, and drug resistance. The new antibody for human RCN is used in the distribution of RCN in normal human organs of fetuses and adults with or without inflammation. Immunohistochemical examination demonstrated a broad distribution of RCN in various organs of fetuses and adults, predominantly in the endocrine and exocrine organs. However, RCN expression was heterogeneous in each constituent cell of some organs. Among non-epithelial organs, vascular endothelial cells, testicular germ cells, neurons, and follicular dendritic cells showed strong staining. Plasma cells were the only RCN-positive cells among hematopoietic and lymphoid cells. In inflammatory conditions, RCN expression was enhanced in both epithelial and non-epithelial cells. Heterogeneous expression of RCN indicates that the amount of RCN needed for cell behavior and life may be variable, depending on each cell type and, therefore, RCN may be helpful in establishing the cell origin of neoplasms in some organs. However, further study is needed to establish the significance of RCN in tumorigenesis and in some peculiar features of neoplasms.