Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species
Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species
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DOI：
10.4269/ajtmh.2003.69.200
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发表时间：
2003-08-01
影响因子：
3.3
通讯作者：
Fontenille, D
中科院分区：
文献类型：
--
作者：
Cohuet, A;Simard, F;Fontenille, D
不死按蚊是非洲疟疾的主要传播媒介。它属于一组兄弟物种，只能在其发育的某些阶段进行形态学鉴定。一种基于聚合酶链反应（PCR）的诊断工具使区分该组中的五个物种成为可能。该测定似乎适用于这些物种中的四个物种的所有分布区域：funestus，安. Leesoni，An. parensis和An. vaneedenip。第五个物种，一个。rivulorum，是第二个最丰富的物种组，并可能被误认为在其成年阶段的主要媒介疟疾。funestus。鉴定为An的标本的分子和形态学观察。来自喀麦隆和南非的rivulorum表明它们属于两个不同的类群。该物种在喀麦隆发现，并在这里命名为An。rivulorum一样，可能会延伸到西非和中非。种特异性PCR测定通过对An特异性的引物补充。rivulorum样，从而有可能区分的五种An。funestus组和新定义的分类单元。
Anopheles funestus is a major vector of malaria in Africa. It belongs to a group of sibling species that can be identified morphologically only at certain stages of their development. A diagnostic polymerase chain reaction (PCR)-based tool made it possible to differentiate five species of the group. The assay seems to be applicable over all their distribution area for four of these species: An. funestus, An. leesoni, An. parensis, and An. vaneedenip. The fifth species, An. rivulorum, is the second most abundant species of the group and can be mistaken at its adult stage for the major vector of malaria An. funestus. Molecular and morphologic observations of specimens identified as An. rivulorum from Cameroon and South Africa showed that they belong to two different taxa. The species identified in Cameroon, and named here An. rivulorum-like, might extend to western Africa and central Africa. The species-specific PCR assay is supplemented by a primer specific to An. rivulorum-like and thus makes it possible to differentiate the five species of the An. funestus group and the newly defined taxon.