Plasmonic Enzyme-Linked Immunosorbent Assay Using Nanospherical Brushes as a Catalase Container for Colorimetric Detection of Ultralow Concentrations of Listeria monocytogenes
Plasmonic Enzyme-Linked Immunosorbent Assay Using Nanospherical Brushes as a Catalase Container for Colorimetric Detection of Ultralow Concentrations of Listeria monocytogenes
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使用纳米球刷作为过氧化氢酶容器的等离激元酶联免疫吸附测定，用于比色检测超低浓度的单核细胞增生李斯特氏菌
DOI：
10.1021/acsami.5b10181
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发表时间：
2015-12-30
影响因子：
9.5
通讯作者：
Li, Yanbin
中科院分区：
文献类型：
--
作者：
Chen, Rui;Huang, Xiaolin;Li, Yanbin
基于过氧化氢酶（CAT）介导的金纳米颗粒生长的等离子体酶联免疫吸附测定（pELISA）表现出使用夹心形式检测疾病相关生物标志物的高灵敏度。然而，该策略对单核细胞增生李斯特菌的检测限（LOD）仅为10（3）CFU/mL左右，这大大超过了李斯特菌的量。食品中常见的单核细胞增多症（
Plasmonic enzyme-linked immunosorbent assay (pELISA) based on catalase (CAT)-mediated gold nanoparticle growth exhibits ultrahigh sensitivity for detecting disease-related biomarkers using sandwich formats. However, the limit of detection (LOD) of this strategy for Listeria monocytogenes is only around 10 (3) CFU/mL, which considerably exceeds the amount of L. monocytogenes commonly present in food products (