EXCITATORY SYNAPTIC ACTION OF CLIMBING FIBRES ON PURKINJE CELLS OF CEREBELLUM
EXCITATORY SYNAPTIC ACTION OF CLIMBING FIBRES ON PURKINJE CELLS OF CEREBELLUM
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DOI：
10.1113/jphysiol.1966.sp007824
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发表时间：
1966-01-01
影响因子：
5.5
通讯作者：
SASAKI, K
中科院分区：
文献类型：
--
作者：
ECCLES, JC;LLINAS, R;SASAKI, K
在猫的副橄榄的刺激选择性地兴奋纤维，具有强大的突触兴奋作用的浦肯野细胞在对侧蚓部，引起重复的尖峰放电的5-7毫秒的持续时间。这种反应几乎无一例外地带有要么全有要么全无的性质。从各个方面来看，它都与攀缘纤维的突触作用相对应。浦肯野细胞的细胞内记录表明，这种攀爬纤维刺激引起了一个大的单一的去极化与初始尖峰和后来的部分尖峰响应叠加在持续的去极化。通过刺激顶核区域的橄榄-小脑通路，可以激发典型的攀爬纤维反应，但选择性要小得多，通常浦肯野细胞的逆行峰电位在前。穿刺浦肯野细胞迅速恶化与损失的所有尖峰放电，攀登纤维的反应，然后减少到兴奋性突触后电位。该电位表明，刺激下橄榄核可在同一攀爬纤维中以约2毫秒的间隔引起2次或更多次放电。下橄榄核神经元连接的复杂性也表明了相当大的反应潜伏期范围。在刺激小脑顶核区域时，最初的直接攀爬纤维反应通常伴随着反射放电，可能来自下橄榄，这类似于下橄榄刺激产生的反应，通常是重复的。典型的攀爬纤维反应是由周围神经刺激引起的，并且经常自发发生。由单个浦肯野细胞中的攀爬纤维引起的反应可以解释下橄榄刺激在大脑皮层表面和通过大脑皮层深度引起的电位场。通过记录细胞内电极的电流的应用，已经有可能影响由攀爬纤维产生的兴奋性突触后电位的大的变化，它被减少，甚至逆转与去极化电流和大大增加超极化电流。
Stimulation in the accessory olive of the cat selectively excites fibers that have a powerful synaptic excitatory action on Purkinje cells in the contralateral vermis, evoking a repetitive spike discharge of 5-7 msec duration. Almost invariably this response had an all-or-nothing character. In every respect it corresponds with the synaptic action that is to be expected from climbing fibers. Intracellular recording from Purkinje cells reveals that this climbing fiber stimulation evokes a large unitary depolarization with an initial spike and later partial spike responses superimposed on a sustained depolarization. Typical climbing fiber responses can be excited, but in a much less selective manner, by stimulation of the olive-cerebellar pathway in the region of the fastigial nucleus, there being often a preceding antidromic spike potential of the Purkinje cell. Impaled Purkinje cells rapidly deteriorate with loss of all spike discharge, the climbing fiber response being then reduced to an excitatory post-synaptic potential. This potential shows that stimulation of the inferior olive may evoke 2 or more discharges at about 2 msec intervals in the same climbing fiber. The complexity of neuronal connexions in the inferior olive is also indicated by the considerable latency range in responses. With stimulation in the region of the fastigial nucleus, the initial direct climbing fiber response is often followed by a reflex discharge, presumably from the inferior olive, which resembles the responses produced by inferior olive stimulation in being often repetitive. Typical climbing fiber responses have been evoked by peripheral nerve stimulation and frequently occur spontaneously. The responses evoked by climbing fibers in the individual Purkinje cells can account for the potential fields that an inferior olive stimulus evokes on the surface and through the depth of the cere-bellar cortex. By the application of currents through the recording intracellular electrode it has been possible to effect large changes in the excitatory post-synaptic potential produced by a climbing fiber, it being diminished and even reversed with depolarizing currents and greatly increased by hyperpolarizing currents.