A sodium-independent mechanism for L-arginine uptake by rat renal brush border membrane vesicles.
A sodium-independent mechanism for L-arginine uptake by rat renal brush border membrane vesicles.
复制标题
大鼠肾刷状缘膜囊泡摄取 L-精氨酸的钠独立机制。
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Poujeol
中科院分区：
文献类型：
--
作者：
T. Jean;P. Ripoche;P. Poujeol
L-精氨酸在肾皮质切片或刷状缘膜囊中的积累不同于中性氨基酸和糖的积累，因为它可以在孵育液中缺乏钠的情况下发生。在不同条件下对L精氨酸的摄取进行了测定，以确定这种转运中钠离子非依赖性成分的相对重要性。D-葡萄糖只有在氯化钠梯度下才能积累到囊泡中，而L精氨酸的积累可被氯化钠、氯化锂、氯化钾或氯胆碱诱导。L-精氨酸和D-葡萄糖的摄取与膜电位成正比(内小于0)，但摄取L-精氨酸不需要Na+。低盐或高盐(Li+或Na+)对L精氨酸摄取的Kj值无明显差异。JMax也在相同的震级范围内。内流D-葡萄糖或L-丙氨酸(Na+-共转运分子)可引起与~(22)Na平衡的囊泡钠含量增加，而摄取L-精氨酸则不能诱导。这些不同的结果表明，电位而不是化学梯度是L精氨酸摄取的主要驱动力。
L-arginine accumulation in renal cortex slices or in brush border membrane vesicles differs from that of neutral amino acids and sugars, since it can occur despite a lack of sodium in the incubation medium. L-arginine uptake was measured under different conditions in order to determine the relative importance of the Na+-independent component of this transport. In contrast to D-glucose, which was accumulated into the vesicles only in the presence of a NaCl gradient, the accumulation of L-arginine was induced by NaCl, LiCl, KCl, or choline Cl gradients. The uptakes of L-arginine and D-glucose wer proportional to the membrane potential (inside less than 0), but the presence of Na+ was not required for the L-arginine uptake. No difference was observed in Kj values of the L-arginine uptake measured with low or high concentration of salt (Li+ or Na+). Jmax were also in the same range of magnitude. An influx of D-glucose or L-alanine (Na+-cotransported molecules) induced an increase of the sodium content in vesicles equilibrated with 22Na; the L-arginine uptake did not induce it. These different results suggest that the electrical potential and not the chemical gradient was the major driving force for the L-arginine uptake.
兔肾刷状缘膜囊泡中不依赖 Na 的 L-精氨酸转运。
DOI：
10.1016/0005-2736(82)90036-0
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hammerman,MR
通讯作者：
Hammerman,MR