CULTURE OF HORMONE-DEPENDENT FUNCTIONAL EPITHELIAL-CELLS FROM RAT THYROIDS
CULTURE OF HORMONE-DEPENDENT FUNCTIONAL EPITHELIAL-CELLS FROM RAT THYROIDS
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DOI：
10.1073/pnas.77.6.3455
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
COON, HG
中科院分区：
文献类型：
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作者：
AMBESIIMPIOMBATO, FS;PARKS, LAM;COON, HG
大鼠甲状腺细胞原代培养在含0.1-0.5%小牛血清和6种激素或生长因子：胰岛素、促甲状腺素、转铁蛋白、氢化可的松、生长抑素和甘氨酰-L-组氨酰-L-赖氨酸醋酸酯的培养基中。FRTL菌株通过连续的菌落分离纯化，并保持高度分化的特征（分泌到培养基中的生理量的甲状腺球蛋白和碘化物浓度的100倍）。FRTL菌株已在连续培养中观察了3年以上。它保持了相同的生化和形态特征，代表了甲状腺滤泡细胞的原代培养物后，立即从大鼠甲状腺酶释放。在更常规的细胞培养条件下生长的甲状腺上皮细胞未能保留这些特化特征。在体外维持这些专门功能的大鼠甲状腺滤泡细胞是依赖于低血清浓度和补充激素的原代培养。这种培养策略可能适用于其他上皮细胞培养中维持分化特征的一般问题。
Primary cultures of rat thyroid cells were made in medium supplented with 0.1-0.5% calf serum and containing 6 hormones or growth factors: insulin, thyrotropin, transferrin, hydrocortisone, somatostatin and glycyl-L-histidyl-L-lysine acetate. The FRTL strain was purified by successive colonial isolations and maintained highly differentiated features (secretion into the culture medium of physiological amounts of thyroglobulin and concentration of iodide by 100-fold). The FRTL strain has been observed for more than 3 yr in continuous culture. It has maintained the same biochemical and morphological characteristics that typified the primary cultures of thyroid follicular cells immediately after their enzymatic release from the rat thyroid. Thyroid epithelial cells that were grown udner more conventional cell culture conditions failed to retain these specialized characteristics. Maintenance in vitro of these specialized functions of rat thyroid follicular cells is dependent on low serum concentrations and supplementation with hormones in the primary cultures. This culture strategem may be applicable to the general problem of maintenance of differentiated characteristics in cultures of other epithelial cells.