STUDY ON PRESERVATION OF BEATING RHYTHM OF SINGLE MYOCARDIAL CELLS IN-VITRO
STUDY ON PRESERVATION OF BEATING RHYTHM OF SINGLE MYOCARDIAL CELLS IN-VITRO
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DOI：
10.1016/0014-4827(73)90316-9
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发表时间：
1973-01-01
影响因子：
3.7
通讯作者：
GOSHIMA, K
中科院分区：
文献类型：
--
作者：
GOSHIMA, K
胎鼠心脏单个心肌细胞在单层培养中自发跳动。在标准培养液中，细胞在37℃下至少保持恒定的搏动频率5h。当单个心肌细胞经EGTA处理后短时间停止搏动，或在低pH条件下孵育细胞减慢搏动频率时，可通过更换原培养液中的细胞来恢复原来的搏动频率。同样的程序也恢复了细胞在低钠高钾离子含量的介质中孵育后受到干扰后的速率。细胞在10℃保存22~24 h后可恢复原来的搏动频率，而37℃保存22~24 h后则不能恢复原来的搏动频率。
Single myocardial cells from fetal mouse heart beat spontaneously in monolayer culture. In standard medium they maintained constant beating rates for at least 5 h at 37 °C. When the beats of single myocardial cells were stopped for a short time by treatment with EGTA, or slowed down by incubating the cells in medium of low pH, the original beating rates could be restored by replacing the cells in the original medium. The same procedure also restored the rates after they had been disturbed by incubating the cells in medium of low sodium and high potassium ion content. Moreover, the original beating rates could be restored after keeping the cells at 10 °C for 22–24 h, but not after keeping them at 37 °C for 22–24 h.