Effects of Platinum Nanocolloid in Combination with Gamma Irradiation on Normal Human Esophageal Epithelial Cells.
Effects of Platinum Nanocolloid in Combination with Gamma Irradiation on Normal Human Esophageal Epithelial Cells.
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DOI：
10.1166/jnn.2016.12362
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发表时间：
2016-05
影响因子：
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通讯作者：
Qiang Li;Yoshiharu Tanaka;Y. Saitoh;N. Miwa
中科院分区：
文献类型：
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作者：
Qiang Li;Yoshiharu Tanaka;Y. Saitoh;N. Miwa
我们之前的研究表明，铂纳米胶体（Pt-nc）与3,5和7 Gy的低剂量γ射线照射联合可显著降低人食管鳞状细胞癌衍生细胞系KYSE-70的增殖并加速其凋亡。本研究的目的是确定，在与我们之前的研究相同的条件下，伽马射线与Pt-nc联合对KYSE-70细胞具有致癌作用，我们是否可以诱导对人类正常食管上皮细胞（HEEpiC）的辐射防护或辐射致敏作用。HEEpiC用不同浓度的Pt-nc处理，然后用不同剂量的伽马射线照射。采用台盼蓝染色法和WST-8法检测HEEpiC的增殖情况。分别用结晶紫染色和Hoechst 33342染色测定细胞和核酸形态学特征。采用硝基蓝四氮唑（NBT）法测定HEEpiC细胞内活性氧（ROS）水平。Western blot检测caspase-3、Bax、Bcl-2的凋亡情况。Pt-nc和γ辐照均能抑制HEEpiC的生长；然而，与单独照射相比，它们的联合使用以Pt-nc剂量依赖的方式发挥了显著的增殖抑制作用。与KYSE-70细胞类似，当Pt-nc单独或与γ辐射联合给药时，Pt-nc对体外HEEpiC产生辐射致敏而不是辐射保护。因此，Pt-nc对细胞增殖具有抑制作用，对细胞凋亡具有促进作用，并对HEEpiC具有一定的毒性。
Our previous study demonstrated that platinum nanocolloid (Pt-nc), combined with lower-dose gamma irradiation at 3, 5, and 7 Gy significantly decreased proliferation and accelerated apoptosis of the human esophageal squamous cell carcinoma-derived cell line KYSE-70. The aim of the present study was to determine, under the same conditions as our previous study where gamma rays combined with Pt-nc were carcinostatic to KYSE-70 cells, if we could induce a radioprotective or the radiation-sensitizing effect on the human normal esophageal epithelial cells (HEEpiC). HEEpiC were treated with various Pt-nc concentrations and then irradiated with various gamma-ray doses. The proliferative status of HEEpiC was evaluated using trypan blue dye-exclusion and WST-8 assays. The cellular and nucleic morphological features were determined using crystal violet and Hoechst 33342 stainings, respectively. The intracellular level of reactive oxygen species (ROS) in HEEpiC was evaluated with a nitro blue tetrazolium (NBT) assay. The apoptotic status was detected with caspase-3, Bax, and Bcl-2 by Western blotting. Either Pt-nc or gamma irradiation could inhibit the growth of HEEpiC; however, their combined use exerted a significant proliferation-inhibitory effect in a Pt-nc dose-dependent manner than gamma irradiation alone. Pt-nc resulted in radiation sensitization rather than radiation protection on HEEpiC in vitro similar to KYSE-70 cells, when Pt-nc was administrated alone or combined with gamma irradiation. Thus, Pt-nc has an inhibitory effect on cell proliferation, a facilitative effect on apoptosis, and a certain degree of toxicity against HEEpiC.