CHLAMYDIA-TRACHOMATIS (L2 SEROVAR) BINDS TO DISTINCT SUBPOPULATIONS OF HUMAN PERIPHERAL-BLOOD LEUKOCYTES
CHLAMYDIA-TRACHOMATIS (L2 SEROVAR) BINDS TO DISTINCT SUBPOPULATIONS OF HUMAN PERIPHERAL-BLOOD LEUKOCYTES
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DOI：
10.1016/0090-1229(86)90134-0
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发表时间：
1986-02-01
期刊：
影响因子：
--
通讯作者：
LEVITT, D
中科院分区：
文献类型：
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作者：
BARD, J;LEVITT, D
我们之前已经证明，患有由沙眼衣原体（一种专性细胞内细菌）引起的肺炎的婴儿，其外周血中 B 淋巴细胞百分比增加，但 T 淋巴细胞百分比没有增加。随后证明，衣原体在体外诱导人外周血 B 淋巴细胞增殖，并且在 T 细胞存在的情况下，诱导 B 细胞分化为免疫球蛋白分泌细胞。在这项研究中，我们发现沙眼衣原体（L2 血清型）优先与外周血中 50% 的人类 B 淋巴细胞结合，但仅与一小部分（如果有的话）T 细胞结合。单核细胞和粒细胞都结合并摄取衣原体。尽管衣原体与 B 细胞结合并被单核细胞摄取，但 B 细胞不摄取并且单核细胞生长有限（含有包涵体的细胞少于 0.5%）。培养后与细菌相关的细胞百分比急剧下降。这些结果首次证明了沙眼衣原体（L2 血清型）与淋巴细胞的结合，并且代表着将细菌和淋巴细胞之间的物理相互作用与体外特异性免疫刺激活性相关联的直接步骤。
We have previously shown that infants with pneumonitis caused by Chlamydia trachomatis, an obligate intracellular bacterium, possess increased percentages of B lymphocytes but not T lymphocytes in their peripheral blood. It was then demonstrated that chlamydiae induce proliferation in vitro of human peripheral blood B lymphocytes and, in the presence of T cells, differentiation of B cells to immunoglobulin-secreting cells. In this study, we show that C. trachomatis (L2 serovar) binds preferentially to 50% of human B lymphocytes from peripheral blood but only to a small percentage, if any, of T cells. Both monocytes and granulocytes bind and ingest chlamydiae. Despite chlamydial binding to B cells and ingestion by monocytes, no uptake by B cells and limited growth (fewer than 0.5% inclusion-containing cells) in monocytes occur. These is a dramatic decrease in the percentage of cells associated with the bacteria after culture. These results are the first demonstration of binding of C. trachomatis (L2 serovar) to lymphocytes and represent a direct step toward correlating physical interactions between bacteria and lymphocytes with specific immunostimulatory activites in vitro.