Atmnd1-delta1 is sensitive to gamma-irradiation and defective in meiotic DNA repair.
Atmnd1-delta1 is sensitive to gamma-irradiation and defective in meiotic DNA repair.
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发表时间：
2006
期刊：
影响因子：
3.8
通讯作者：
Séverine Domenichini;Cécile Raynaud;D. Ni;Y. Henry;C. Bergounioux
中科院分区：
文献类型：
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作者：
Séverine Domenichini;Cécile Raynaud;D. Ni;Y. Henry;C. Bergounioux
基因组DNA中双链断裂（DSB）的有效修复对所有生物的生存至关重要。Mnd 1被认为在减数分裂重组过程中促进链侵入步骤。我们使用了正向遗传学的方法，通过寻找突变体，表征Mnd 1的拟南芥同源物。atmnd 1无效突变体表现出正常的营养体和花发育。相反，在前期I，染色体变得片段化，导致片段在多分体之间随机分布。雄性和雌性减数分裂有缺陷，AtRAD 51在atmnd 1-delta 1核中观察到强烈的积累。这些结果表明，类似于它的酵母和动物同系物，AtMnd 1在减数分裂期间的DSB修复中发挥作用。此外，与其人类同源物AtMnd 1一样，AtMnd 1在体细胞中表达。AtMnd 1的表达强烈诱导的γ射线和无效突变体显示增加的敏感性，γ射线。综上所述，这些结果表明，AtMnd 1也是体细胞中DSB修复所必需的。
The efficient repair of double-strand breaks (DSBs) in genomic DNA is crucial for the survival of all organisms. Mnd1 is suggested to promote the strand invasion step during meiotic recombination. We used a forward genetics approach, through the search for mutants, to characterize the Arabidopsis homologue of Mnd1. Atmnd1 null mutants exhibit normal vegetative and flower development. In contrast, during prophase I, chromosomes become fragmented resulting in random distribution of the fragments between polyads. Male and female meiosis are defective and strong accumulation of AtRAD51 was observed in atmnd1-delta1 nuclei. These results suggest that similarly to its yeast and animal homologues, AtMnd1 plays a role in DSB repair during meiosis. In addition, like its human homologue AtMnd1 is expressed in somatic cells. AtMnd1 expression is strongly induced by gamma-rays and null mutants show increased sensibility to gamma-rays. Taken together, these results suggest that AtMnd1 is also required for DSB repair in somatic cells.