Determination of benzidine, N-acetylbenzidine, and N,N'-diacetylbenzidine in human urine by capillary gas chromatography negative ion chemical ionization mass spectrometry
Determination of benzidine, N-acetylbenzidine, and N,N'-diacetylbenzidine in human urine by capillary gas chromatography negative ion chemical ionization mass spectrometry
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DOI：
10.1006/abio.1996.0070
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发表时间：
1996-02-15
影响因子：
2.9
通讯作者：
Davis, B
中科院分区：
文献类型：
--
作者：
Hsu, FF;Lakshmi, V;Davis, B
本文报道了用毛细管气相色谱/负离子化学电离同位素稀释质谱法定量尿中联苯胺（BZ）及其乙酰化代谢物N-乙酰联苯胺（ABZ）和N，N '-二乙酰联苯胺（DABZ）的方法。尿样经固相萃取柱纯化，用LiAlH 4/THF还原，用五氟丙酸酐衍生。通过选择离子监测相对于氘标记的内标物测量衍生物。BZ、ABZ和DABZ的检测限分别低至0.5、0.8和1.5 ppt。在接触BZ或BZ染料的工人中，ABZ的尿浓度大大超过BZ或DABZ。该方法已成功地用于测量BZ，ABZ，和DABZ在1.0毫升尿样收集参与制造BZ和BZ染料的工人。该方法也适用于其他芳香胺及其乙酰化代谢物的测定。(C)出版社：Academic Press，Inc.
We report an isotope dilution mass spectrometry method using capillary gas chromatography/negative ion chemical ionization to quantitate urine concentrations of benzidine (BZ) and of its acetylated metabolites N-acetylbenzidine (ABZ) and N,N'-diacetylbenzidine (DABZ). Urine samples were purified by solid-phase extraction columns, reduced with LiAlH4/THF, and derivatized with pentafluoropropionic anhydride. The derivatives were measured by selected ion monitoring relative to deuterium-labeled internal standards. A detection limit as low as 0.5, 0.8, and 1.5 ppt for BZ, ABZ, and DABZ, respectively, can easily be achieved. Urinary concentrations of ABZ substantially exceed those of either BZ or of DABZ in workers exposed to BZ or BZ-based dyes. This method has been successfully used to measure BZ, ABZ, and DABZ in 1.0-ml urine samples collected from workers involved in manufacturing BZ and BZ-based dyes. The method should be applicable to the measurement of other aromatic amines and their acetylated metabolites. (C) 1996 Academic Press, Inc.