Neck collar for restraining head and body movements in rats for behavioral task performance and simultaneous neural activity recording.
Neck collar for restraining head and body movements in rats for behavioral task performance and simultaneous neural activity recording.
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颈圈用于限制大鼠的头部和身体运动，以执行行为任务并同时记录神经活动。
DOI：
10.1016/j.jneumeth.2016.02.005
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Tsutsui KI
中科院分区：
文献类型：
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作者：
Tateyama Y;Oyama K;Lo CW;Iijima T;Tsutsui KI
背景头部固定是行为神经生理学的主要方法之一，因为它可以在刺激应用和行为评估中提供精确的信息。大多数清醒猴子的神经记录都是在头部固定的情况下获得的，目前这种方法也被用于清醒的啮齿动物。然而，大鼠头部固定装置往往在几个月内变得不稳定，从而增加了骨骼和周围组织炎症、感染和坏死的风险。新方法本研究开发了一种新型的非侵入性“颈圈系统”，用于限制行为大鼠头和身体的运动。结果颈圈固定2-3个月不影响动物的健康和福利。颈圈固定的大鼠可以学习一项行为任务(标准延迟舔舔任务)，其学习效率与标准头部固定相同。他们还可以学习一个更复杂的任务(延迟亲舔/反舔任务)，然后将他们的学习迁移到标准头部固定下的任务上。此外，在标准延迟舔食任务中，我们能够记录颈项固定下的大鼠的单位活动。与现有的方法(S)相比，该系统由经济的材料组成，易于构建，并且无需手术就可以实现头部约束，从而消除了炎症或感染的风险。结论我们认为本研究开发的颈项固定系统可以用于约束行为啮齿类动物的头部。
BackgroundHead fixation has been one of the major methods in behavioral neurophysiology because it allows precision in stimulus application and behavioral assessment. Most neural recordings in awake monkeys have been obtained under head fixation, which is nowadays also being used in awake rodents. However, head fixation devices in rats often become unstable within several months, which increases risks for inflammation, infection, and necrosis of the bone and surrounding tissue.New methodIn this study we developed a novel non-invasive “neck collar system” for restraining the head and body movements of behaving rats.ResultsThe attachment of the neck collar for 2–3 months did not affect the animals’ health and welfare. Rats under neck-collar fixation could learn a behavioral task (standard delayed licking task) with the same efficiency as those under standard head fixation. They could also learn a more complicated task (delayed pro/anti-licking task) under neck-collar fixation and afterwards transfer their learning to the task under standard head fixation. Furthermore, we were able to record single-unit activity in rats under neck-collar fixation during the performance of the standard delayed licking task.Comparison with existing method(s)This system consists of economical materials and is easily constructed, and it enables head-restraint without surgery, thus eliminating the risk of inflammation or infection.ConclusionsWe consider the neck-collar fixation developed in this study would be useful for restraining the head of a behaving rodent.