Genetic characteristics of blaNDM-1-positive plasmid in Citrobacter freundii isolate separated from a clinical infectious patient
Genetic characteristics of blaNDM-1-positive plasmid in Citrobacter freundii isolate separated from a clinical infectious patient
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DOI：
10.1099/jmm.0.057091-0
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Yu, Yun-Song
中科院分区：
文献类型：
--
作者：
Du, Xiao-Xing;Wang, Jian-Feng;Yu, Yun-Song
本研究报告了一例涉及产NDM-1的弗氏柠檬酸杆菌的感染病例，并通过分析NDM-1编码质粒序列的特征，进一步探讨了bla（NDM-1）基因的潜在威胁。bla（NDM-1）阳性C.对碳青霉烯类抗生素具有高耐药性的弗氏菌是从患有尿路感染的临床患者中分离的。S1核酸酶为基础的质粒分析，然后Southern印迹杂交，接合实验和电转化证实，bla（NDM-1）基因位于质粒上。对bla（NDM-1）阳性质粒（pCFNDM-CN）的高通量测序结果表明，该质粒为54 kb IncX型质粒，含有一个主链区和一个可变区，并含有两个β-内酰胺酶基因（bla（NDM-1）和bla（SHV-12））。NDM-1复合转座子在可变区被IS 26和IS 5截短的ISAba 125包围，与不动杆菌属的bla（NDM-1）包围结构具有高度的序列相似性。我们的研究表明，NDM-1复合转座子可能在不动杆菌bla（NDM-1）基因的动员中发挥重要作用。肠杆菌科
This study reports an infectious case involving an NDM-1-producing Citrobacter freundii and further explored the potential threat of the bla(NDM-1) gene by analysing the characteristics of the NDM-1-encoding plasmid sequence. A bla(NDM-1)-positive C. freundii with high resistance to carbapenems was separated from a clinical patient suffering from a urinary tract infection. S1 nuclease-based plasmid analysis followed by Southern blot hybridization, a conjugation experiment and electrotransformation confirmed that the bla(NDM-1) gene was located on a plasmid. High-throughput sequencing of the bla(NDM-1)-postive plasmid (pCFNDM-CN) showed that it was a 54 kb IncX-type plasmid and contained a backbone region and a variable region with two beta-lactamase genes (bla(NDM-1) and bla(SHV-12)). The NDM-1 composite transposon in the variable region was surrounded by IS26 and IS5-truncated ISAba125, and shared a high sequence similarity to the bla(NDM-1) surrounding structure in Acinetobacter spp. Our research suggested that the NDM-1 composite transposon might play an essential role in mobilization of the bla(NDM-1) gene from Acinetobacter spp. to Enterobacteriaceae.