Selected factors affecting Staphylococcus aureus within silastic catheters.
Selected factors affecting Staphylococcus aureus within silastic catheters.
复制标题
影响硅橡胶导管内金黄色葡萄球菌的选定因素。
DOI：
10.1016/j.jss.2009.07.025
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Hess,DonavonJ
中科院分区：
文献类型：
--
作者：
Henry-Stanley,MichelleJ;Shepherd,MelodyM;Wells,CarolL;Hess,DonavonJ
导管相关感染是住院患者常见的并发症，金黄色葡萄球菌是常见的病原体。对影响S.材料和方法体外实验评估了S.金黄色葡萄球菌粘附在硅橡胶导管上。还评估了肝素、血清和钙对初始细菌粘附的影响。另外的实验量化了乙醇锁定对S.金黄色葡萄球菌孵育48至72小时后产生的导管相关生物膜内的金黄色葡萄球菌活力。金黄色葡萄球菌与导管的条件下的营养流。扫描电子显微镜观察了乙醇锁定对粘附在导管壁上的细菌赘生物形态的影响。金黄色葡萄球菌容易粘附（以剂量依赖性方式）到与细菌一起孵育15分钟的硅橡胶导管上，并且粘附不受钙或肝素的影响（即使肝素粘附到硅橡胶管和金黄色葡萄球菌。已知金黄色葡萄球菌表达肝素结合蛋白）。S.金黄色葡萄球菌粘附被血清而不是白蛋白抑制。含成熟48 - 72 h S的导管的乙醇锁定（5 min-24 h）。金黄色葡萄球菌生物膜导致没有可检测到的细菌活力，尽管扫描电子显微镜显示类似的细菌赘生物粘附到对照和乙醇处理的导管上。金黄色葡萄球菌对硅橡胶导管的粘附被血清抑制，但活性抑制成分不是白蛋白。乙醇封闭有效灭菌S。金黄色葡萄球菌污染的导管，虽然无活力的细菌赘生物仍然在乙醇处理的导管。
BACKGROUNDCatheter-related infections are frequent complications in hospitalized patients, and Staphylococcus aureus is a frequent etiologic agent. Little is known about factors that contribute to the growth and viability of S. aureus within contaminated catheters.MATERIALS AND METHODSIn vitro experiments assessed the ability of S. aureus to adhere to silastic catheter tubing. The effects of heparin, serum, and calcium on initial bacterial adherence were also assessed. Additional experiments quantified the effect of ethanol locking on S. aureus viability within catheter-associated biofilms produced after 48 to 72 h incubation of S. aureus with catheters under conditions of nutrient flow. Scanning electron microscopy visualized the effect of ethanol locking on the morphology of bacterial vegetations adherent to the catheter wall.RESULTSS. aureus readily adhered (in a dose dependent manner) to silastic catheters incubated with bacteria for 15min, and adherence was not affected by calcium or heparin (even though heparin adhered to the silastic tubing and S. aureus is known to express heparin-binding proteins). S. aureus adherence was inhibited by serum but not albumin. Ethanol locking (5 min to 24 h) of catheters containing mature 48 to 72h S. aureus biofilms resulted in no detectable bacterial viability, although scanning electron microscopy revealed similar bacterial vegetations adherent to control and ethanol-treated catheters.CONCLUSIONS. aureus adherence to silastic catheters was inhibited by serum, but the active inhibitory component was not albumin. Ethanol locking efficiently sterilized S. aureus contaminated catheters, although nonviable bacterial vegetations remained on the ethanol-treated catheters.