Imaging of the pulmonary circulation in the closed-chest rat using synchrotron radiation microangiography.
Imaging of the pulmonary circulation in the closed-chest rat using synchrotron radiation microangiography.
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DOI：
10.1152/japplphysiol.00596.2006
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发表时间：
2007-02
影响因子：
3.3
通讯作者：
D. Schwenke;J. Pearson;K. Umetani;K. Kangawa;M. Shirai
中科院分区：
文献类型：
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作者：
D. Schwenke;J. Pearson;K. Umetani;K. Kangawa;M. Shirai
肺动脉高压发病过程中肺循环的结构变化尚未完全阐明。虽然血管造影术已被用于显示肺循环，但传统的血管造影术在显示小血管(直径200微米)方面有相当大的局限性，尤其是在闭胸动物模型中。在这项研究中，我们评估了单色同步辐射(SR)对完整胸腔大鼠肺循环的微血管造影的有效性。成年雄性SD大鼠麻醉后，在右室内放置导管，注射碘化造影剂(Imer On 350)。随后，使用单色SR对左肺内的肺动脉分支进行微血管成像。此外，我们评估了急性低氧(10%和8%O2各4分钟)肺血管收缩(HPV)过程中血管直径的动态变化。使用SR，我们能够看到直径为100微米的肺微血管(从左轴动脉发出的第四代分支)。急性缺氧导致所有直径小于500微米的血管显著减少。直径在200-300微米之间的血管肺血管收缩程度最大。这些结果证明了SR在显示闭胸大鼠模型中的肺血管和评估与HPV相关的动态变化方面的有效性。更重要的是，这些观察结果表明，SR是未来评估与肺动脉高压发病机制相关的大体结构变化的有效工具。
Structural changes of the pulmonary circulation during the pathogenesis of pulmonary arterial hypertension remain to be fully elucidated. Although angiography has been used for visualizing the pulmonary circulation, conventional angiography systems have considerable limitations for visualizing small microvessels (diameters < 200 microm), particularly within a closed-chest animal model. In this study we assess the effectiveness of monochromatic synchrotron radiation (SR) for microangiography of the pulmonary circulation in the intact-chest rat. Male adult Sprague-Dawley rats were anesthetized, and a catheter was positioned within the right ventricle, for administering iodinated contrast agent (Iomeron 350). Subsequently, microangiography of pulmonary arterial branches within the left lung was performed using monochromatic SR. Additionally, we assessed dynamic changes in vessel diameter during acute hypoxic (10% and 8% O2 for 4 min each) pulmonary vasoconstriction (HPV). Using SR we were able to visualize pulmonary microvessels with a diameter of <100 microm (the 4th generation of branching from the left axial artery). Acute hypoxia caused a significant decrease in the diameter of all vessels less than 500 microm. The greatest degree of pulmonary vasoconstriction was observed in vessels with a diameter between 200 and 300 microm. These results demonstrate the effectiveness of SR for visualizing pulmonary vessels in a closed-chest rat model and for assessing dynamic changes associated with HPV. More importantly, these observations implicate SR as an effective tool in future research for assessing gross structural changes associated with the pathogenesis of pulmonary arterial hypertension.