Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly
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DOI：
10.1126/science.1060118
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发表时间：
2001-04-06
期刊：
影响因子：
56.9
通讯作者：
Grewal, SIS
中科院分区：
文献类型：
--
作者：
Nakayam, J;Rice, JC;Grewal, SIS
高阶染色质结构的组装已与组蛋白尾巴的共价修饰有关。我们提供体内证据，表明组蛋白H3（H3 LYS（9））的赖氨酸9被裂变酵母中异染色质相关区域的ClR4蛋白优先甲基化。 H3 LYS（9）体内甲基化都需要保守的CLR4的铬和集合域。 SWI6的定位是果蝇HP1的同源物与异缘区域的同源物，取决于H3 Lysg甲基化。此外，通过CLR4和SWI6定位，需要H3特异性的脱乙酰基酶CLR3和β-螺旋桨结构域蛋白Rik1（9）甲基化。这些数据定义了一个保守的途径，其中顺序组蛋白修饰为异染色质组装的表观遗传遗传所必需的“组蛋白代码”建立了“组蛋白代码”。
The assembly of higher order chromatin structures has been Linked to the covalent modifications of histone tails. We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys(9)) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions in fission yeast. Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys(9) methylation in vivo. Localization of Swi6, a homolog of Drosophila HP1, to heterochomatic regions is dependent on H3 Lysg methylation. Moreover, an H3-specific deacetylase Clr3 and a beta -propeller domain protein Rik1 are required for H3 Lys(9) methylation by Clr4 and Swi6 Localization. These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly.