Effects of combined stretching and clenbuterol on disuse atrophy in rat soleus muscle.
Effects of combined stretching and clenbuterol on disuse atrophy in rat soleus muscle.
复制标题
联合拉伸和克伦特罗对大鼠比目鱼肌废用性萎缩的影响。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
K. Tachino
中科院分区：
文献类型：
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作者：
T. Yamazaki;M. Yokogawa;K. Tachino
背景和目的
临床上，废用性肌肉萎缩常见于严重虚弱且长期卧床的患者。在危重患者的医疗治疗早期，常见的物理治疗干预措施无法成功预防废用性肌肉萎缩。在这种情况下，使用 β2-肾上腺素能激动剂如克伦特罗 (Cb) 可能有助于预防萎缩。此外，最近的研究表明，拉伸可以预防废用性肌肉萎缩和肌肉力量下降。本研究的目的是评估废用性肌肉萎缩过程中 Cb 药物联合拉伸 (ST) 对大鼠比目鱼肌 (SOL) 的影响。
科目
本研究使用了 35 只雄性 Wistar 大鼠。
方法
将大鼠分为五组：对照组（CON）、仅后肢减重（HU）组、HU+ST、HU+Cb 药物组和 HU+ST+Cb 组。拉伸组中的右侧SOL每天通过在非麻醉下被动背屈踝关节来维持拉伸位置一小时。实验时间为2周。
结果
在ST组中，比目鱼肌单位横截面积的峰值抽搐张力显着大于Cb组，而CON组和ST组之间没有显着差异。在 Cb 组中观察到的 I 型纤维向 II 型纤维的转变在 ST 和 Cb 组合组中没有被识别到。
讨论与结论
统计上并未认识到联合拉伸和 Cb 药物治疗的显着效果。结果表明，Cb 影响肌肉形态特征，而拉伸影响收缩特性。这些数据表明，考虑到肌纤维类型特异性的 ST 和 Cb 联合干预可能需要更多考虑，以防止废用性肌肉萎缩和肌力下降。
BACKGROUND AND PURPOSE
Clinically, disuse muscle atrophy is often seen among patients who are severely debilited and are on prolonged bed rest. Common physical therapy interventions are not successful in preventing disuse muscle atrophy early in the medical treatment of critically ill patients. In situations such as this, the use of a β 2-adrenergic agonist such as clenbuterol (Cb) may be of benefit in preventing atrophy. Also, recent studies have suggested that stretching is possible in preventing disuse muscle atrophy and the decline in muscle strength. The objective of this study was to evaluate the effects of Cb medication combined with stretching (ST) on rat soleus muscle (SOL) during the progression of disuse muscle atrophy.
SUBJECTS
Thirty-five male Wistar rats were used in this study.
METHODS
The rats were divided into five groups: control (CON), hindlimb-unweighting (HU) only, HU+ST, HU+Cb medication, and HU+ST+Cb groups. The right SOL in stretching groups was maintained a stretched position for one hour daily by passively dorsiflexing the ankle joint under non-anesthesia. The experimental period was 2 weeks.
RESULTS
In the ST group, peak twitch tension per cross-sectional area in soleus muscle was significantly larger than in the Cb group, while there was no significant difference between the CON and ST groups. The conversion of type I to type II fibers that was observed in the Cb group was not recognized in the combined ST and Cb group.
DISCUSSION AND CONCLUSION
Distinct effect of combined stretching and Cb medication was not recognized statistically. The results indicate that Cb affects muscle morphological characteristics while stretching affects contractile properties. These data suggest that a combined ST and Cb intervention considered the type-specificity of muscle fiber may be need more consideration for preventing disuse muscle atrophy and the decline in muscle strength.
克伦特罗（一种 β2 受体激动剂）引起骨骼肌纤维从慢到快的变化。
DOI：
10.1152/ajpendo.1988.254.6.e726
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
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作者：
Zeman,RJ;Ludemann,R;Easton,TG;Etlinger,JD
通讯作者：
Etlinger,JD