Quantifying the relationship between surfaces' nano-contact point density and adhesion force of Candida albicans
Quantifying the relationship between surfaces' nano-contact point density and adhesion force of Candida albicans
复制标题
DOI：
10.1016/j.colsurfb.2020.111177
复制
发表时间：
2020-10-01
影响因子：
5.8
通讯作者：
Jandt, Klaus D.
中科院分区：
文献类型：
--
作者：
Dauben, Thomas J.;Dewald, Carolin;Jandt, Klaus D.
最近已经认识到，在纳米尺度上的受控表面结构化是赋予不同材料抗菌性能的成功策略。尽管有许多关于细菌与纳米结构表面相互作用的研究，但表面形貌和细菌粘附之间的定量联系仍然缺失。为了定量地链接细胞粘附数据与地形表面参数，我们进行了单细胞光谱化学上相同的表面与控制纳米接触点密度实现固定的金纳米粒子（AuNP）的金薄膜。此类材料表面先前已显示出对革兰氏阴性大肠杆菌细胞的抗微生物（抗粘附）功效。在目前的研究中，纳米结构表面对临床相关的白色念珠菌（念珠菌）的表面覆盖和粘附力的影响。白色念珠菌），主要与植入物感染相关的真菌，以验证它们对不同微生物细胞的抗微生物效力。C.白色念珠菌细胞与纳米结构表面的接触显示出随着接触点密度的降低而降低的趋势，并且与相应的C.白色念珠菌细胞计数。具有最低接触点密度（25 AuNP/μ m（2））的表面导致5 nN的平均粘附力，与对照和61 AuNP/μ m（2）表面相比，该平均粘附力低达5倍。此外，对力-距离曲线的详细分析表明，粘附功以及去除C。与非结构化表面相比，在25 AuNP/μ m（2）表面上从表面分离白色念珠菌细胞的能力最多低10倍。这些研究结果表明，可控调谐的纳米结构表面的可访问的纳米接触点是至关重要的，以产生具有增强的抗菌性能的表面结构。所获得的知识可以进一步用于生物材料表面的设计，以防止一些最常见的病原体的粘附。
It has been recently recognized that controlled surface structuring on the nanometer scale is a successful strategy to endow different materials with antimicrobial properties. Despite many studies on bacterial interactions with nanostructured surfaces, a quantitative link between surface topography and bacterial adhesion is still missing. To quantitatively link cell adhesion data with topographical surface parameters, we performed single-cell spectroscopy on chemically identical surfaces with controlled nano-contact point density achieved by immobilization of gold nanoparticles (AuNP) on gold thin films. Such materials surfaces have previously shown antimicrobial (anti-adhesive) efficacy towards Gram-negative Escherichia coli cells. In the current study, the influence of nano-structured surfaces on the surface coverage and adhesion forces of clinically relevant Candida albicans (C. albicans), the fungus primarily associated with implant infections, was investigated to validate their antimicrobial potency against different microbial cells. The adhesion forces of C. albicans cells to nanostructured surfaces showed a decreasing trend with decreasing contact-point density and correlated well with the results of the respective C. albicans cell counts. The surfaces with the lowest contact-point density, 25 AuNP/mu m(2), resulted in an average adhesion force of 5 nN, which was up to 5 times lower compared to control and 61 AuNP/mu m(2) surfaces. Further, detailed analyses of force-distance curves revealed that the work of adhesion, and thus the energy required to remove the C. albicans cell from the surface is up to 10 times lower on 25 AuNP/mu m(2) surfaces compared to unstructured surfaces. These findings show that a controlled tuning of nanostructured surfaces in terms of accessible nano-contact points is crucial to generate surface structures with enhanced antimicrobial properties. The gained knowledge can be further exploited for the design of biomaterials surfaces to prevent adhesion of some most commonly encountered pathogens.