EFFECTS OF IRRADIANCE AND METHYL VIOLOGEN TREATMENT ON ATP, ADP, AND ACTIVATION OF RIBULOSE BISPHOSPHATE CARBOXYLASE IN SPINACH LEAVES
EFFECTS OF IRRADIANCE AND METHYL VIOLOGEN TREATMENT ON ATP, ADP, AND ACTIVATION OF RIBULOSE BISPHOSPHATE CARBOXYLASE IN SPINACH LEAVES
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DOI：
10.1104/pp.88.3.850
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发表时间：
1988-11-01
期刊：
影响因子：
7.4
通讯作者：
SHARKEY, TD
中科院分区：
文献类型：
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作者：
BROOKS, A;PORTIS, AR;SHARKEY, TD
由于核酮糖二磷酸羧化酶（Rubisco）在体外对ATP和ADP敏感，本研究旨在探讨菠菜完整叶片中ATP水平与Rubisco活化状态的相关性。响应于辐照度的变化并且在供给电子受体甲基紫精之后。叶暴露于各种照射45分钟，在大气分压的CO2和O2。在测量CO2同化率后，将叶片原位冷冻夹持，并对穿孔圆盘进行Rubisco活性测定，以及核酮糖二磷酸（RuBP）、ATP和ADP的量。光合速率和Rubisco的活化状态随光强的增加而增加，但RuBP、ATP和ADP的水平没有受到太大的影响。甲基紫精喂养的叶片在低光照下有93%的rubisco活化状态相比，在对照叶片的51%。叶片的ATP含量也显著更高，并且ATP与ADP的比率在甲基紫精饲喂的叶片中为4.1，而在对照叶片中为2.2。从这些结果和其他已发表的结果，我们得出结论，ATP水平和Rubisco活化之间的相关性，可以观察到在完整的叶片，但在辐照度的变化过程中，一些额外的因素参与调节Rubisco活化。
Since activation of ribulose bisphosphate carboxylase (rubisco) by rubisco activase is sensitive to ATP and ADP in vitro, we aimed to test the correlation between ATP level and rubisco activation state in intact leaves of Spinacia oleracea L. in response to changes in irradiance and after feeding the electron acceptor methyl viologen. Leaves were exposed to various irradiances for 45 minutes at atmospheric partial pressures of CO2 and O2. After measuring the rate of CO2 assimilation, leaves were freeze-clamped in situ and the punched discs assayed for rubisco activity, and amounts of ribulose bisphosphate (RuBP), ATP, and ADP. The photosynthetic rate and the activation state of rubisco increased with increasing irradiance but the levels of RuBP, ATP, and ADP were not greatly affected. Methyl viologen fed leaves under low irradiance had rubisco activation states of 93% compared to 51% in control leaves. The ATP content of the leaves was also significantly higher and the ratio of ATP to ADP was 4.1 in methyl viologen fed leaves compared to 2.2 in control leaves. From these results and other published results we conclude that a correlation between ATP level and rubisco activation can be observed in intact leaves, but that during changes in irradiance some additional factors are involved in regulating rubisco activation.