Live-Cell Fluorescence Imaging for Virus-Host Interactions.
Live-Cell Fluorescence Imaging for Virus-Host Interactions.
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用于病毒-宿主相互作用的活细胞荧光成像。
DOI：
10.1007/978-1-0716-3617-6_3
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Hyser,JosephM
中科院分区：
文献类型：
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作者：
Scribano,FrancescaJ;Engevik,KristenA;Gebert,JThomas;Hyser,JosephM
最近显微镜技术的进步促进了研究宿主-病原体相互作用的新方法。特别是，显微镜硬件和工程生物传感器的改进有助于克服荧光显微镜对活细胞成像的障碍。活体荧光显微镜允许检测离散信号事件和蛋白质定位，提高我们评估药物，微生物或感染的影响的能力，具有高时间分辨率。在这里，我们描述了一个方案的长期活细胞荧光成像的病毒感染细胞系。
Recent technological advances in microscopy have facilitated novel approaches to investigate host-pathogen interactions. In particular, improvements in both microscope hardware and engineered biosensors have helped to overcome barriers to live-cell imaging with fluorescence microscopy. Live fluorescent microscopy allows for the detection of discrete signaling events and protein localization, improving our ability to assess the effects of pharmacologic agents, microbes, or infection with high temporal resolution. Here we describe a protocol for long-term live-cell fluorescence imaging of virus infected cell lines.