Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.
Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity.
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DOI：
10.1371/journal.pone.0134893
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Iossa S
中科院分区：
文献类型：
--
作者：
Di Luccia B;Crescenzo R;Mazzoli A;Cigliano L;Venditti P;Walser JC;Widmer A;Baccigalupi L;Ricca E;Iossa S
富含果糖的饮食会诱发代谢综合征，这是一种健康紊乱的组合，会增加患糖尿病和心血管疾病的风险。饮食也被认为会改变肠道的微生物组成，尽管尚不清楚这种改变是否有助于代谢综合征的发展。这项工作的目的是评估肠道微生物群与肥胖大鼠模型中饮食诱导的代谢综合征发展之间的可能联系。大鼠被喂食标准或高果糖饮食。通过口服管饲法，用抗生素或来自对照大鼠的粪便样品处理果糖喂养的大鼠组。测量各组动物的身体成分、血浆代谢参数和组织氧化应激标志物。采用16S DNA测序方法对不同日粮条件下动物肠道细菌组成进行了研究。富含果糖的饮食诱导了代谢综合征、炎症和氧化应激的标志物，当动物接受抗生素或粪便样本治疗时，这些标志物都显著降低。两个细菌属，粪球菌属和瘤胃球菌属的成员的数量，增加了富含果糖的饮食和抗生素和粪便治疗减少，指出它们的丰度和代谢综合征的发展之间的相关性。我们的数据表明，在大鼠喂养的果糖丰富的饮食代谢综合征的发展是直接相关的特定细菌类群的肠道内容物的变化。
A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.